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Wnt-8a Antibody (HL1648) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-25755

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-25755

Key Product Details

Species Reactivity

Validated:

Human, Zebrafish

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1648

Format

Azide and BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for Wnt-8a Antibody (HL1648) - Azide and BSA Free

Immunogen

Recombinant fragment of human Wnt-8a

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Wnt-8a Antibody (HL1648) - Azide and BSA Free

Wnt-8a Antibody (HL1648) - Azide and BSA Free

Immunohistochemistry-Paraffin: Wnt-8a Antibody (HL1648) - Azide and BSA Free [NBP3-25755] -

Immunohistochemistry-Paraffin: Wnt-8a Antibody (HL1648) - Azide and BSA Free [NBP3-25755] - Wnt8a antibody [HL1648] detects Wnt8a protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human colon cancer. Wnt8a stained by Wnt8a antibody [HL1648] (NBP3-25755) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
Wnt-8a Antibody (HL1648) - Azide and BSA Free

Western Blot: Wnt-8a Antibody (HL1648) - Azide and BSA Free [NBP3-25755] -

Western Blot: Wnt-8a Antibody (HL1648) - Azide and BSA Free [NBP3-25755] - Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with Wnt8a antibody [HL1648] (NBP3-25755) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Wnt-8a Antibody (HL1648) - Azide and BSA Free

Western Blot: Wnt-8a Antibody (HL1648) - Azide and BSA Free [NBP3-25755] -

Western Blot: Wnt-8a Antibody (HL1648) - Azide and BSA Free [NBP3-25755] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt8a antibody [HL1648] (NBP3-25755) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for Wnt-8a Antibody (HL1648) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:1000-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Wnt-8a

The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family, and may be implicated in development of early embryos as well as germ cell tumors. It encodes a protein which shows 81% amino acid identity to the mouse Wnt8A protein. [provided by RefSeq]

Long Name

Wingless-type MMTV Integration Site Family, Member 8a

Alternate Names

Wnt8a

Gene Symbol

WNT8A

Additional Wnt-8a Products

Product Documents for Wnt-8a Antibody (HL1648) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Wnt-8a Antibody (HL1648) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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