WRCH1 Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-93157

Novus Biologicals, part of Bio-Techne
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NBP2-93157-0.02ml
NBP2-93157-0.1ml
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all WRCH1 Antibodies »

Product Summary for WRCH1 Antibody - Azide and BSA Free

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 40-100 of human WRCH1 (NP_067028.1). GRAGGAEGRGVKCVLVGDGAVGKTSLVVSYTTNGYPTEYIPTAFDNFSAVVSVDGRPVRLQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WRCH1 Antibody - Azide and BSA Free

Western Blot: WRCH1 AntibodyAzide and BSA Free [NBP2-93157]

Western Blot: WRCH1 AntibodyAzide and BSA Free [NBP2-93157]

Western Blot: WRCH1 Antibody [NBP2-93157] - Analysis of extracts of U-87MG cells, using WRCH1 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.

Applications for WRCH1 Antibody - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.01% Thimerosal

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: WRCH1

Function: Acts upstream of PAK1 to regulate the actin cytoskeleton, adhesion turnover and increase cell migration. Stimulates quiescent cells to reenter the cell cycle. Has no detectable GTPase activity but its high intrinsic guanine nucleotide exchange activity suggests it is constitutively GTP-bound.; Subcellular location: Cell membrane; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor. Cell junction > focal adhesion. Cell projection > podosome. Note: Localizes to podosomes in SRC-transformed cells.; Tissue specificity: Ubiquitously expressed in all tissues examined. Expressed at high levels in the stomach, small intestine, brain, skeletal muscle and placenta.

Alternate Names

ARHU, CDC42L1GTP-binding protein-like 1, DJ646B12.2, fJ646B12.2, FLJ10616,2310026M05Rik, G28K, GTP-binding protein like 1, GTP-binding protein SB128, hG28K, ras homolog gene family, member U, ras-like gene family member U, Rho GTPase-like protein ARHU, rho-related GTP-binding protein RhoU, Ryu GTPase, Wnt-1 responsive Cdc42 homolog 1, WRCH-1, WRCH1CDC42-like GTPase 1

Gene Symbol

RHOU

Additional WRCH1 Products

Product Documents for WRCH1 Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for WRCH1 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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