WRN Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31895

Novus Biologicals, part of Bio-Techne
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NBP1-31895
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all WRN Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human WRN. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

162 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for WRN Antibody

Western Blot: WRN Antibody [NBP1-31895]

Western Blot: WRN Antibody [NBP1-31895]

Western Blot: WRN Antibody [NBP1-31895] - Sample (30 ug of whole cell lysate) A: A431 5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: WRN Antibody [NBP1-31895]

Immunocytochemistry/ Immunofluorescence: WRN Antibody [NBP1-31895]

Immunocytochemistry/Immunofluorescence: WRN Antibody [NBP1-31895] - Paraformaldehyde-fixed HeLa, using WRN antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with an alpha Tubulin antibody (Red) at 1:2500.
Immunohistochemistry-Paraffin: WRN Antibody [NBP1-31895]

Immunohistochemistry-Paraffin: WRN Antibody [NBP1-31895]

Immunohistochemistry-Paraffin: WRN Antibody [NBP1-31895] - Human normal colon. WRN antibody dilution: 1:250. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Applications for WRN Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Application Notes
IP-Assay dependent
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: WRN

This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging.

Long Name

Werner syndrome ATP-dependent helicase

Alternate Names

EC 3.1.-.-, Exonuclease WRN, RecQ protein-like 2, RecQ3, RECQL2

Gene Symbol

WRN

Additional WRN Products

Product Documents for WRN Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for WRN Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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