Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 960522

Concentration

1.0 mg/ml

View all WT1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human WT1
Met127-Gly249
Accession # P19544

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for WT1 Antibody (960522)

Immunocytochemistry: WT1 Antibody (960522) [MAB5729]

Immunocytochemistry: WT1 Antibody (960522) [MAB5729]

Immunocytochemistry: WT1 Antibody (960522) [MAB5729] - WT-1 was detected in immersion fixed K-562 cell line using MAB5729 at 8 ug/ml for 3 hours at room temperature. Cells were stained using NL007 (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplam & nuclei.
Flow Cytometry: WT1 Antibody (960522) [MAB5729]

Flow Cytometry: WT1 Antibody (960522) [MAB5729]

Flow Cytometry: WT1 Antibody (960522) [MAB5729] - An intracellular stain was performed on THP-1 cells with WT1 [960522] Antibody MAB5729AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.

Applications for WT1 Antibody (960522)

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is Cytof ready.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: WT1

Wilms' tumor protein (WT1) is a transcription factor which regulates the expression of numerous target genes, including EPO. This protein plays an essential role in the normal development of the urogenital system, including cellular development and cell survival, and it has both tumor suppressor and oncogenic roles in tumor formation.

Defects in the WT1 protein can lead to Wilms tumor, Frasier syndrome, Denys-Drash syndrome, nephrotic syndrome type 4, and Meacham syndrome.

Long Name

Wilms Tumor 1

Alternate Names

GUD, WAGR, WIT-2, WT33

Gene Symbol

WT1

Additional WT1 Products

Product Documents for WT1 Antibody (960522)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for WT1 Antibody (960522)

0

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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