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WTAP Antibody (PCRP-WTAP-1A4) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23995

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # PCRP-WTAP-1A4

Format

Azide and BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant full-length human WTAP protein

Localization

Nucleus. Nucleolus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Antibody without azide - store at -20 to -80C.

Scientific Data Images for WTAP Antibody (PCRP-WTAP-1A4) - Azide and BSA Free

Protein Array: WTAP Antibody (PCRP-WTAP-1A4) - Azide and BSA Free [NBP3-23995] -

Analysis of Protein Array containing more than 19,000 full-length human proteins using WTAP antibody (PCRP-WTAP-1A4) - Azide and BSA Free

Flow Cytometry: WTAP Antibody (PCRP-WTAP-1A4) - Azide and BSA Free [NBP3-23995] -

Flow Cytometric Analysis of PFA-fixed HeLa cells. WTAP antibody (PCRP-WTAP-1A4) - Azide and BSA Free followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

Immunohistochemistry-Paraffin: WTAP Antibody (PCRP-WTAP-1A4) - Azide and BSA Free [NBP3-23995] -

Formalin-fixed, paraffin-embedded human colon carcinoma stained with WTAP antibody (PCRP-WTAP-1A4) - Azide and BSA Free. HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Applications for WTAP Antibody (PCRP-WTAP-1A4) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1-2ug/million cells

Immunohistochemistry-Paraffin

1-2ug/ml
Application Notes
Immunohistochemistry-Paraffin: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: WTAP

The Wilms tumor suppressor gene WT1 appears to play a role in both transcriptional and posttranscriptional regulation of certain cellular genes. This gene encodes a WT1-associating protein, which is a ubiquitously expressed nuclear protein. Like WT1 protein, this protein is localized throughout the nucleoplasm as well as in speckles and partially colocalizes with splicing factors. Alternative splicing of this gene results in three transcript variants, two of which encode the same isoform. [provided by RefSeq]

Alternate Names

Female-lethal(2)D homolog, hFL(2)D, KIAA0105DKFZp686F20131, MGC3925, PNAS-132, pre-mRNA-splicing regulator WTAP, putative pre-mRNA splicing regulator female-lethal(2D), Wilms tumor 1 associated protein, Wilms tumor 1-associating protein, Wilms' tumour 1-associating protein, WT1-associated protein

Gene Symbol

WTAP

Additional WTAP Products

Product Documents for WTAP Antibody (PCRP-WTAP-1A4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for WTAP Antibody (PCRP-WTAP-1A4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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