WW domain binding protein 5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84208

Novus Biologicals, part of Bio-Techne
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NBP1-84208
NBP1-84208-25ul
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all WW domain binding protein 5 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: EGKPENESEPKHEEEPKPEEKPEEEEKLEEEAKAKGTFRERLIQSLQEFKEDIHNRHLSNEDMFREVDEIDEIRRVRNKLIVMRWKVNRN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for WW domain binding protein 5 Antibody

Western Blot: WW domain binding protein 5 Antibody [NBP1-84208]

Western Blot: WW domain binding protein 5 Antibody [NBP1-84208]

Western Blot: WW domain binding protein 5 Antibody [NBP1-84208] - Analysis in control (vector only transfected HEK293T lysate) and WBP5 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: WW domain binding protein 5 Antibody [NBP1-84208]

Immunocytochemistry/ Immunofluorescence: WW domain binding protein 5 Antibody [NBP1-84208]

Immunocytochemistry/Immunofluorescence: WW domain binding protein 5 Antibody [NBP1-84208] - Staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
Immunohistochemistry-Paraffin: WW domain binding protein 5 Antibody [NBP1-84208]

Immunohistochemistry-Paraffin: WW domain binding protein 5 Antibody [NBP1-84208]

Immunohistochemistry-Paraffin: WW domain binding protein 5 Antibody [NBP1-84208] - Staining of human thyroid gland shows cytoplasmic positivity in glandular cells.

Applications for WW domain binding protein 5 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: WW domain binding protein 5

The globular WW domain is composed of 38 to 40 semiconserved amino acids shared by proteins of diverse functions including structural, regulatory, and signaling proteins. The domain is involved in mediating protein-protein interactions through the binding of polyproline ligands. This gene encodes a WW domain binding protein. This gene also encodes a domain with similarity to the transcription elongation factor A, SII-related family. Alternative splicing results in multiple transcript variants encoding a single isoform. Transcript Variant: This variant (1) represents the longest transcript. Variants 1 through 4 encode the same isoform.

Alternate Names

DKFZp313K1940, N4, NDFIP1, pp21 homolog, TCEAL9, WBP-5, WW domain binding protein 1, WW domain binding protein 5, WW domain-binding protein 5

Gene Symbol

TCEAL9

Additional WW domain binding protein 5 Products

Product Documents for WW domain binding protein 5 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for WW domain binding protein 5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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