XAGE1D Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-997

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all XAGE1D Antibodies »

Product Specifications

Immunogen

Peptide with sequence CGFGFRRQGEDNT corresponding to C-Terminus according to NP_597673.1.

Specificity

This antibody is expected to recognise isoform d (NP_597673.1) only. Reported variants represent identical protein (NP_597673.1; NP_001091067.1; NP_001091074.1; NP_001091062.1; NP_001091065.1).

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for XAGE1D Antibody

Immunohistochemistry-Paraffin: XAGE1D Antibody [NB300-997]

Immunohistochemistry-Paraffin: XAGE1D Antibody [NB300-997]

Immunohistochemistry-Paraffin: XAGE1D Antibody [NB300-997] - (3.8ug/ml) IHC staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for XAGE1D Antibody

Application
Recommended Usage

Immunohistochemistry

3 - 6 ug/ml

Immunohistochemistry-Paraffin

3 - 6 ug/ml

Peptide ELISA

Detection limit 1:8000
Application Notes
WB: Not yet tested - Our standard WB protocol would not expect to reliably detect proteins as small as the predicted MW of approx. 8.0 kDa band according to NP_597673. IHC-P: Human testis shows polar cytoplasm staining in primary spermatocytes.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: XAGE1D

This gene is a member of the XAGE subfamily, which belongs to the GAGE family. The GAGE genes are expressed in a variety of tumors and in some fetal and reproductive tissues. This gene is strongly expressed in Ewing's sarcoma, alveolar rhabdomyosarcoma and normal testis. The protein encoded by this gene contains a nuclear localization signal and shares a sequence similarity with other GAGE/PAGE proteins. Because of the expression pattern and the sequence similarity, this protein also belongs to a family of CT (cancer-testis) antigens. Alternative splicing of this gene generates 3 transcript variants, and one of which includes 2 transcripts generated from alternate transcription initiation sites.

Alternate Names

cancer/testis associated protein, CT12.1C, CTP9, G antigen family D member 2, member 1c, X antigen family, member 1C, XAGE1

Entrez Gene IDs

9503 (Human)

Gene Symbol

XAGE1D

UniProt

Q9HD64 (Human)

Additional XAGE1D Products

Product Documents for XAGE1D Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for XAGE1D Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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