XIAP Antibody (S02-9A3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15111

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15111-100ul
NBP3-15111-25ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S02-9A3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all XIAP Antibodies »

Product Summary for XIAP Antibody (S02-9A3)

Immunogen

Recombinant protein of human XIAP (Uniprot#: Q60989)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for XIAP Antibody (S02-9A3)

Western Blot: XIAP Antibody (S02-9A3) [NBP3-15111]

Western Blot: XIAP Antibody (S02-9A3) [NBP3-15111]

Western Blot: XIAP Antibody (S02-9A3) [NBP3-15111] - Western blot detection of XIAP in K562, Rat Brain, 3T3 cell lysates using XIAP Rabbit mAb (1:1000 diluted). Predicted band size: 57kDa. Observed band size: 53kDa.

Applications for XIAP Antibody (S02-9A3)

Application
Recommended Usage

Western Blot

1/1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: XIAP

XIAP [human X-linked IAP, hILP (human IAP-like protein), MIHA, BIRC4) is a member of the family of inhibitor of apoptosis proteins (IAP). IAPs suppress mitochondria-dependent and independent apoptosis by binding to and inhibiting caspases through their BIR domains (reviewed in Liston et al, 2003; Wright and Duckett, 2005). Resistance towards apoptosis is a hallmark of cancer cells, and overexpression of IAPs can contribute to the development of cancer though inhibiting apoptosis. In addition to at least one BIR domain, some IAP members also have a RING-type finger motif at their carboxyl-terminal. The RING finger domain of several IAPs, including XIAP, have E3 ubiquitin ligase activity and target the degradation of Smac/DIABLO through ubqiuitination (Morizane et al, 2005). Smac/DIABLO is a death inducer and functions by inhibiting IAP-caspase interactions, thereby promoting apoptosis. Degradation of cell death inducers like Smac/DIABLO is thought to be a conserved mechanism by which IAPs enhance their anti-apoptotic activity, thereby promoting cell survival. XIAP is highly characterized with respect to its structure and biochemical mechanisms, and has received interest as a therapeutic target (reviewed in Schimmer, 2006). Since XIAP blocks a substantial portion of the apoptosis pathway and is associated with chemoresistance in cancer cells, inhibiting XIAP has been a focus for potential therapeutics. Approaches have included antisense oligonucleotides and small molecule inhibitors. Small molecules that that target the BIR2 and BIR3 domains of XIAP are considered particularly attractive. This is because the BIR domains inhibit caspase activity, and it is thought that removing the inhibition should increase the cell's ability to undergo apoptosis as well as decrease its potential for chemoresistance. Full-length human XIAP is a 497 amino acid protein and migrates at approx. 53 kDa on SDS-PAGE.

Long Name

X-linked Inhibitor of Apoptosis

Alternate Names

BIRC4

Gene Symbol

XIAP

Additional XIAP Products

Product Documents for XIAP Antibody (S02-9A3)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for XIAP Antibody (S02-9A3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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