XK X-linked Kx blood group Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05963
![Western Blot: XK X-linked Kx blood group Antibody [NBP3-05963]](https://resources.bio-techne.com/images/products/XK-X-linked-Kx-blood-group-Antibody-Western-Blot-NBP3-05963-img0001.jpg "Western Blot: XK X-linked Kx blood group Antibody [NBP3-05963]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for XK X-linked Kx blood group Antibody
Immunogen
Produced in rabbits immunized with E. coli-derived Human XK X-linked Kx blood group fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for XK X-linked Kx blood group Antibody
Western Blot: XK X-linked Kx blood group Antibody [NBP3-05963]
Western Blot: XK X-linked Kx blood group Antibody [NBP3-05963] - Lane A: MCF7 Whole Cell LysateLane B: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:51 kDa(We are unsure as to the identity of these extra bands.)![Immunocytochemistry/ Immunofluorescence: XK X-linked Kx blood group Antibody [NBP3-05963]](https://resources.bio-techne.com/images/products/XK-X-linked-Kx-blood-group-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-05963-img0002.jpg "Immunocytochemistry/ Immunofluorescence: XK X-linked Kx blood group Antibody [NBP3-05963]")
Immunocytochemistry/ Immunofluorescence: XK X-linked Kx blood group Antibody [NBP3-05963]
Immunocytochemistry/Immunofluorescence: XK X-linked Kx blood group Antibody [NBP3-05963] - Staining of XK in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human XK polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.Applications for XK X-linked Kx blood group Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: XK X-linked Kx blood group
Alternate Names
Kell blood group precursor (McLeod phenotype), Kell complex 37 kDa component, Kx, Kx antigen, membrane transport protein XK, X1k, XK, Kell blood group complex subunit (McLeod syndrome), XKR1XK-related protein 1, X-linked Kx blood group (McLeod syndrome), XRG1
Gene Symbol
XK
Additional XK X-linked Kx blood group Products
Product Documents for XK X-linked Kx blood group Antibody
Product Specific Notices for XK X-linked Kx blood group Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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