XLF Antibody (OTI3D6)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03275

Novus Biologicals, part of Bio-Techne
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NBP2-03275
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Key Product Details

Species Reactivity

Validated:

Human, Monkey

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3D6

Concentration

0.52 mg/ml

View all XLF Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human NHEJ1 (NP_079058) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

33.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for XLF Antibody (OTI3D6)

Western Blot: XLF Antibody (OTI3D6) [NBP2-03275]

Western Blot: XLF Antibody (OTI3D6) [NBP2-03275]

Western Blot: XLF Antibody (3D6) [NBP2-03275] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XLF (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XLF.
Immunocytochemistry/ Immunofluorescence: XLF Antibody (OTI3D6) [NBP2-03275]

Immunocytochemistry/ Immunofluorescence: XLF Antibody (OTI3D6) [NBP2-03275]

Immunocytochemistry/Immunofluorescence: XLF Antibody (3D6) [NBP2-03275] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY XLF.
Immunohistochemistry-Paraffin: XLF Antibody (OTI3D6) [NBP2-03275]

Immunohistochemistry-Paraffin: XLF Antibody (OTI3D6) [NBP2-03275]

Immunohistochemistry-Paraffin: XLF Antibody (3D6) [NBP2-03275] - Staining of paraffin-embedded Human prostate tissue using anti-XLF mouse monoclonal antibody.
View 11 more images

Applications for XLF Antibody (OTI3D6)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:1000-2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.52 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: XLF

Double-strand breaks in DNA result from genotoxic stresses and are among the most damaging of DNA lesions. This gene encodes a DNA repair factor essential for the nonhomologous end-joining pathway, which preferentially mediates repair of double-stranded breaks. Mutations in this gene cause different kinds of severe combined immunodeficiency disorders. [provided by RefSeq].

Alternate Names

Cernunnos, non-homologous end-joining factor 1, nonhomologous end-joining factor 1, Protein cernunnos, XLFFLJ12610, XRCC4-like factor

Entrez Gene IDs

79840 (Human)

Gene Symbol

NHEJ1

Additional XLF Products

Product Documents for XLF Antibody (OTI3D6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for XLF Antibody (OTI3D6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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