XPA Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-93321

Novus Biologicals, part of Bio-Techne
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NB100-93321
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all XPA Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human xeroderma pigmentosum, complementation group A using the numbering given in entry NP_000371.1 (GeneID 7507).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for XPA Antibody

Western Blot: XPA Antibody [NB100-93321]

Western Blot: XPA Antibody [NB100-93321]

Western Blot: XPA Antibody [NB100-93321] - Whole cell lysate (50 ug) from HeLa, K-562, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-XPA antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Immunohistochemistry-Paraffin: XPA Antibody [NB100-93321]

Immunohistochemistry-Paraffin: XPA Antibody [NB100-93321]

Immunohistochemistry-Paraffin: XPA Antibody [NB100-93321] - Detection of human XPA by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- XPA (NB100-93321). Detection: DAB.
Immunoprecipitation: XPA Antibody [NB100-93321]

Immunoprecipitation: XPA Antibody [NB100-93321]

Immunoprecipitation: XPA Antibody [NB100-93321] - Detection of human XPA by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-XPA antibody NB100-93321 (lot NB100-93321-3) used for IP at 6 ug per reaction. XPA was also immunoprecipitated by a previous lot of this antibody (lot NB100-93321-2) and another rabbit anti-XPA antibody. For blotting immunoprecipitated XPA, NB100-93321 was used at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for XPA Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: XPA

This gene encodes a zinc finger protein involved in DNA excision repair. The encoded protein is part of the NER (nucleotide excision repair) complext which is responsible for repair of UV radiation-induced photoproducts and DNA adducts induced by chemical carcinogens. Mutations in this gene are associated with xeroderma pigmentosum complementation group A. [provided by RefSeq]

Long Name

Xeroderma Pigmentosum, Complementation Group A

Alternate Names

XP1

Entrez Gene IDs

7507 (Human)

Gene Symbol

XPA

UniProt

NP_000371.1 (Human)

Additional XPA Products

Product Documents for XPA Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for XPA Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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