XPF Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-38510

Novus Biologicals, part of Bio-Techne
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NBP3-38510-100ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all XPF Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 513-782 of human XPF (NP_005227.1).

Sequence:
GKPLRVYFLIYGGSTEEQRYLTALRKEKEAFEKLIREKASMVVPEEREGRDETNLDLVRGTASADVSTDTRKAGGQEQNGTQQSIVVDMREFRSELPSLIH

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

104 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for XPF Antibody

XPF Antibody

Western Blot: XPF Antibody [NBP3-38510] -

Western Blot: XPF Antibody [NBP3-38510] - Western blot analysis of various lysates using XPF Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
XPF Antibody

Immunoprecipitation: XPF Antibody [NBP3-38510] -

Immunoprecipitation: XPF Antibody [NBP3-38510] - Immunoprecipitation analysis of 150 ug extracts of 293T cells using 3 ug XPF antibody. Western blot was performed from the immunoprecipitate using XPF antibody at a dilution of 1:1000.

Applications for XPF Antibody

Application
Recommended Usage

Immunoprecipitation

0.5ug - 4ug antibody for 200ug - 400ug extracts of whole cells

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: XPF

XPF/ERCC4 is suggested to play a role in the repair of DNA double-strand breaks (DSB), homologous recombination, and gene conversion via single-strand annealing (SSA). XPF/ERCC4 is an endonuclease that incises 5-prime DNA. Defects in XPF/ERCC4 cause xeroderma pigmentosum VI (XP6) an autosomal recessive disease characterized by hypersensitivity to sunlight and a predisposition to skin cancer as well as neurological abnormalities. Defects in XPF/ERCC4 are also responsible for XFE progeroid syndrome, a syndrome characterized by dwarfism, cachexia, and microcephaly.

Long Name

Xeroderma Pigmentosum F

Alternate Names

ERCC4

Gene Symbol

ERCC4

Additional XPF Products

Product Documents for XPF Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for XPF Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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