XPG Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-74611
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 650 and 700 of human excision repair cross-complementing rodent repair deficiency, complementation group 5 (xeroderma pigmentosum, complementation group G (Cockayne syndrome)) (NP_
Localization
Nuclear
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for XPG Antibody
![Western Blot: XPG Antibody [NB100-74611]](https://resources.bio-techne.com/images/products/XPG-Antibody-Western-Blot-NB100-74611-img0010.jpg "Western Blot: XPG Antibody [NB100-74611]")
Western Blot: XPG Antibody [NB100-74611]
Western Blot: XPG Antibody [NB100-74611] - Detection of human ERCC5/XPG by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, HeLa, and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ERCC5/XPG antibody NB100-74611 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunohistochemistry-Paraffin: XPG Antibody [NB100-74611]](https://resources.bio-techne.com/images/products/XPG-Antibody-Immunohistochemistry-Paraffin-NB100-74611-img0011.jpg "Immunohistochemistry-Paraffin: XPG Antibody [NB100-74611]")
Immunohistochemistry-Paraffin: XPG Antibody [NB100-74611]
Immunohistochemistry-Paraffin: XPG Antibody [NB100-74611] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-ERCC5/XPG used at a dilution of 1:200 (1ug/ml). Detection: DAB![Immunoprecipitation: XPG Antibody [NB100-74611]](https://resources.bio-techne.com/images/products/XPG-Antibody-Immunoprecipitation-NB100-74611-img0009.jpg "Immunoprecipitation: XPG Antibody [NB100-74611]")
Immunoprecipitation: XPG Antibody [NB100-74611]
Immunoprecipitation: XPG Antibody [NB100-74611] - Detection of human ERCC5/XPG by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ERCC5/XPG antibody NB100-74611 used for IP at 6 ug per reaction. For blotting immunoprecipitated ERCC5/XPG, NB100-74611 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.Applications for XPG Antibody
Application
Recommended Usage
Immunohistochemistry
1:100-1:500
Immunohistochemistry-Paraffin
1:100-1:500
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Use in ICC/IF reported in scientific literature (PMID:33693861). Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: XPG
Long Name
Xeroderma Pigmentosum G
Alternate Names
ERCC5
Entrez Gene IDs
2073 (Human)
Gene Symbol
ERCC5
UniProt
Additional XPG Products
Product Documents for XPG Antibody
Product Specific Notices for XPG Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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