XPV/DNA polymerase eta Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-60424
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human
Cited:
Rabbit
Predicted:
Mouse (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 690 and 713 of human polymerase (DNA directed), eta using the numbering given in entry NP_006493.1 (GeneID 5429).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
78 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for XPV/DNA polymerase eta Antibody
![Western Blot: XPV/DNA polymerase eta Antibody [NB100-60424]](https://resources.bio-techne.com/images/products/XPV-DNA-polymerase-eta-Antibody-Western-Blot-NB100-60424-img0005.jpg "Western Blot: XPV/DNA polymerase eta Antibody [NB100-60424]")
Western Blot: XPV/DNA polymerase eta Antibody [NB100-60424]
Western Blot: XPV/DNA polymerase eta Antibody [NB100-60424] - Detection of human PolH by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified Rabbit anti-PolH antibody NB100-60424 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.![Immunoprecipitation: XPV/DNA polymerase eta Antibody [NB100-60424]](https://resources.bio-techne.com/images/products/XPV-DNA-polymerase-eta-Antibody-Immunoprecipitation-NB100-60424-img0006.jpg "Immunoprecipitation: XPV/DNA polymerase eta Antibody [NB100-60424]")
Immunoprecipitation: XPV/DNA polymerase eta Antibody [NB100-60424]
Immunoprecipitation: XPV/DNA polymerase eta Antibody [NB100-60424] - Detection of human XPV/DNA polymerase eta/PolH by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hela cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit anti-PolH antibody NB100-60424 (lot 3) used for IP at 6 ug per reaction. PolH was also immunoprecipitated by a previous lot of this antibody (lot 2) and rabbit anti-PolH antibody NB100-60423. For blotting immunoprecipitated PolH, NB100-60424 was used at 0.1 ug/ml. Chemiluminescence with an exposure time of 3 minutes.Applications for XPV/DNA polymerase eta Antibody
Application
Recommended Usage
Immunoprecipitation
2-10 ug/mg lysate
Western Blot
1:2000 -1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: XPV
Long Name
Xeroderma Pigmentosum Variant Type Protein
Alternate Names
DNA Polymerase, eta, POLH, RAD30A
Entrez Gene IDs
5429 (Human)
Gene Symbol
POLH
UniProt
Additional XPV Products
Product Documents for XPV/DNA polymerase eta Antibody
Product Specific Notices for XPV/DNA polymerase eta Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...