Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2F8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all XRCC1 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human XRCC1 (NP_006288) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

69.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for XRCC1 Antibody (OTI2F8) - Azide and BSA Free

Western Blot: XRCC1 Antibody (OTI2F8)Azide and BSA Free [NBP2-74891]

Western Blot: XRCC1 Antibody (OTI2F8)Azide and BSA Free [NBP2-74891]

Western Blot: XRCC1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-74891] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC1.
Immunocytochemistry/ Immunofluorescence: XRCC1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-74891]

Immunocytochemistry/ Immunofluorescence: XRCC1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-74891]

Immunocytochemistry/Immunofluorescence: XRCC1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-74891] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY XRCC1.
Immunohistochemistry: XRCC1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-74891]

Immunohistochemistry: XRCC1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-74891]

Immunohistochemistry: XRCC1 Antibody (OTI2F8) - Azide and BSA Free [NBP2-74891] - Staining of paraffin-embedded pancreas tissue using anti-XRCC1 mouse monoclonal antibody.
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Applications for XRCC1 Antibody (OTI2F8) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: XRCC1

XRCC1 (X-ray cross complementing factor-1) is involved in DNA base excision repair. XRCC1 is a 70kDa protein that has been shown to be physically associated with other DNA repair enzymes, including poly (ADP-ribose) polymerase (PARP), DNA Ligase III and DNA polymerase beta. XRCC1 contains a BRCT domain (for BRCA1 carboxyl terminus) which was originally found in the tumor suppressor protein BRCA1. Recently, the BRCT domain has been shown to mediate the protein-protein interaction of XRCC1 and DNA Ligase III-a.

Alternate Names

DNA repair protein XRCC1, RCC, X-ray repair complementing defective repair in Chinese hamster cells 1, X-ray repair cross-complementing protein 1, X-ray-repair, complementing defective, repair in Chinese hamster

Gene Symbol

XRCC1

Additional XRCC1 Products

Product Documents for XRCC1 Antibody (OTI2F8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for XRCC1 Antibody (OTI2F8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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