Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all XRCC1 Antibodies »

Product Summary for XRCC1 [p Ser461] Antibody

Immunogen

The immunogen recognized by this antibody maps to a region of surrounding residue Ser 461 of human X-ray Repair Complementing protein 1 using the numbering given in SwissProt entry P18887 (GeneID 7515).

Modification

p Ser461

Specificity

NB100-533 is specific for phospho serine human XRCC1 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for XRCC1 [p Ser461] Antibody

Immunohistochemistry: XRCC1 [p Ser461] Antibody [NB100-533]

Immunohistochemistry: XRCC1 [p Ser461] Antibody [NB100-533]

Immunohistochemistry: XRCC1 [p Ser461] Antibody [NB100-533] - Samples: FFPE serial sections of human larynx squamous cell carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho XRCC1 (S461). used at a dilution of 1:1
Immunohistochemistry-Paraffin: XRCC1 [p Ser461] Antibody [NB100-533]

Immunohistochemistry-Paraffin: XRCC1 [p Ser461] Antibody [NB100-533]

Immunohistochemistry-Paraffin: XRCC1 [p Ser461] Antibody [NB100-533] - FFPE serial sections of human breast adenocarcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho XRCC1 (S461). Antibody: Affinity purified rabbit anti-Phospho XRCC1 (S461). used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Ki
Immunohistochemistry-Paraffin: XRCC1 [p Ser461] Antibody [NB100-533]

Immunohistochemistry-Paraffin: XRCC1 [p Ser461] Antibody [NB100-533]

Immunohistochemistry-Paraffin: XRCC1 [p Ser461] Antibody [NB100-533] - Mouse Phospho XRCC1 (S461). Sample: FFPE section of mouse squamous cell carcinoma. Antibody: Affinity purified rabbit anti-Phospho XRCC1 (S461) dilution-1:250.

Applications for XRCC1 [p Ser461] Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:500 - 1:2000

Western Blot

1:1000 -1:5000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: XRCC1

XRCC1 (X-ray cross complementing factor-1) is involved in DNA base excision repair. XRCC1 is a 70kDa protein that has been shown to be physically associated with other DNA repair enzymes, including poly (ADP-ribose) polymerase (PARP), DNA Ligase III and DNA polymerase beta. XRCC1 contains a BRCT domain (for BRCA1 carboxyl terminus) which was originally found in the tumor suppressor protein BRCA1. Recently, the BRCT domain has been shown to mediate the protein-protein interaction of XRCC1 and DNA Ligase III-a.

Alternate Names

DNA repair protein XRCC1, RCC, X-ray repair complementing defective repair in Chinese hamster cells 1, X-ray repair cross-complementing protein 1, X-ray-repair, complementing defective, repair in Chinese hamster

Entrez Gene IDs

7515 (Human)

Gene Symbol

XRCC1

UniProt

P18887 (Human)

Additional XRCC1 Products

Product Documents for XRCC1 [p Ser461] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for XRCC1 [p Ser461] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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