Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all XRCC1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region of surrounding residue Ser 461 of human X-ray Repair Complementing protein 1 using the numbering given in SwissProt entry P18887 (GeneID 7515).

Modification

p Ser461

Specificity

NB100-533 is specific for phospho serine human XRCC1 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for XRCC1 [p Ser461] Antibody

Immunohistochemistry: XRCC1 [p Ser461] Antibody [NB100-533]

Immunohistochemistry: XRCC1 [p Ser461] Antibody [NB100-533]

Immunohistochemistry: XRCC1 [p Ser461] Antibody [NB100-533] - Samples: FFPE serial sections of human larynx squamous cell carcinoma. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Affinity purified rabbit anti-Phospho XRCC1 (S461). used at a dilution of 1:1
Immunohistochemistry-Paraffin: XRCC1 [p Ser461] Antibody [NB100-533]

Immunohistochemistry-Paraffin: XRCC1 [p Ser461] Antibody [NB100-533]

Immunohistochemistry-Paraffin: XRCC1 [p Ser461] Antibody [NB100-533] - FFPE serial sections of human breast adenocarcinoma. Mock phosphatase treated section (left) or calf intestinal phosphatase-treated section (right) immunostained for Phospho XRCC1 (S461). Antibody: Affinity purified rabbit anti-Phospho XRCC1 (S461). used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Ki
Immunohistochemistry-Paraffin: XRCC1 [p Ser461] Antibody [NB100-533]

Immunohistochemistry-Paraffin: XRCC1 [p Ser461] Antibody [NB100-533]

Immunohistochemistry-Paraffin: XRCC1 [p Ser461] Antibody [NB100-533] - Mouse Phospho XRCC1 (S461). Sample: FFPE section of mouse squamous cell carcinoma. Antibody: Affinity purified rabbit anti-Phospho XRCC1 (S461) dilution-1:250.

Applications for XRCC1 [p Ser461] Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:500 - 1:2000

Western Blot

1:1000 -1:5000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: XRCC1

XRCC1 (X-ray cross complementing factor-1) is involved in DNA base excision repair. XRCC1 is a 70kDa protein that has been shown to be physically associated with other DNA repair enzymes, including poly (ADP-ribose) polymerase (PARP), DNA Ligase III and DNA polymerase beta. XRCC1 contains a BRCT domain (for BRCA1 carboxyl terminus) which was originally found in the tumor suppressor protein BRCA1. Recently, the BRCT domain has been shown to mediate the protein-protein interaction of XRCC1 and DNA Ligase III-a.

Alternate Names

DNA repair protein XRCC1, RCC, X-ray repair complementing defective repair in Chinese hamster cells 1, X-ray repair cross-complementing protein 1, X-ray-repair, complementing defective, repair in Chinese hamster

Entrez Gene IDs

7515 (Human)

Gene Symbol

XRCC1

UniProt

P18887 (Human)

Additional XRCC1 Products

Product Documents for XRCC1 [p Ser461] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for XRCC1 [p Ser461] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.