Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all XRCC1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region of surrounding residues Ser 485 and Thr 488 of human X-ray Repair Complementing protein 1 using the numbering given in SwissProt entry P18887 (GeneID 7515).

Modification

p Ser485, p Thr488

Specificity

NB100-541 is specific for human phospho-XRCC1 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for XRCC1 [p Ser485, p Thr488] Antibody

Western Blot: XRCC1 [p Ser485, p Thr488] Antibody [NB100-541]

Western Blot: XRCC1 [p Ser485, p Thr488] Antibody [NB100-541]

Western Blot: XRCC1 [p Ser485, p Thr488] Antibody [NB100-541] - Whole cell lysate (50 ug) from asynchronously growing HeLa (H), 293T (T) and mouse NIH-3T3 (M) cells. Antibody used at 0.1 ug/ml. The binding of the antibody was blocked by pre-incubation with the phospho-peptide but was not blocked by pre-incubation with
Immunohistochemistry-Paraffin: XRCC1 [p Ser485, p Thr488] Antibody [NB100-541]

Immunohistochemistry-Paraffin: XRCC1 [p Ser485, p Thr488] Antibody [NB100-541]

Immunohistochemistry-Paraffin: XRCC1 [p Ser485, p Thr488] Antibody [NB100-541] - Sample: FFPE sections of human stomach carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- Phospho-XRCC1-S485/T488 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB

Applications for XRCC1 [p Ser485, p Thr488] Antibody

Application
Recommended Usage

Immunohistochemistry

1:2000 - 1:10000

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:1000-1:5000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: XRCC1

The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. This protein interacts with DNA ligase III, polymerase beta and poly (ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

Alternate Names

DNA repair protein XRCC1, RCC, X-ray repair complementing defective repair in Chinese hamster cells 1, X-ray repair cross-complementing protein 1, X-ray-repair, complementing defective, repair in Chinese hamster

Entrez Gene IDs

7515 (Human)

Gene Symbol

XRCC1

UniProt

P18887 (Human)

Additional XRCC1 Products

Product Documents for XRCC1 [p Ser485, p Thr488] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for XRCC1 [p Ser485, p Thr488] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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