Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4H9

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all XRCC4 Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human XRCC4 expression vector

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

38.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for XRCC4 Antibody (OTI4H9) - Azide and BSA Free

Western Blot: XRCC4 Antibody (OTI4H9)Azide and BSA Free [NBP2-74896]

Western Blot: XRCC4 Antibody (OTI4H9)Azide and BSA Free [NBP2-74896]

Western Blot: XRCC4 Antibody (OTI4H9) - Azide and BSA Free [NBP2-74896] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY XRCC4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-XRCC4.
Immunohistochemistry: XRCC4 Antibody (OTI4H9) - Azide and BSA Free [NBP2-74896]

Immunohistochemistry: XRCC4 Antibody (OTI4H9) - Azide and BSA Free [NBP2-74896]

Immunohistochemistry: XRCC4 Antibody (OTI4H9) - Azide and BSA Free [NBP2-74896] - Staining of paraffin-embedded lung tissue using anti-XRCC4 mouse monoclonal antibody.
Immunohistochemistry: XRCC4 Antibody (OTI4H9) - Azide and BSA Free [NBP2-74896]

Immunohistochemistry: XRCC4 Antibody (OTI4H9) - Azide and BSA Free [NBP2-74896]

Immunohistochemistry: XRCC4 Antibody (OTI4H9) - Azide and BSA Free [NBP2-74896] - Staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-XRCC4 mouse monoclonal antibody.
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Applications for XRCC4 Antibody (OTI4H9) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:50

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: XRCC4

XRCC4 is encoded by this gene functions together with DNA ligase IV and the DNA-dependent protein kinase in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. The non-homologous end-joining pathway is required both for normal development and for suppression of tumors. This gene functionally complements XR-1 Chinese hamster ovary cell mutant, which is impaired in DNA double-strand breaks produced by ionizing radiation and restriction enzymes. Alternative transcription initiation and alternative splicing generates several transcript variants. [provided by RefSeq]

Alternate Names

complementing defective, repair in Chinese hamster, X-ray repair complementing defective repair in Chinese hamster cells 4, X-ray repair cross-complementing protein 4

Gene Symbol

XRCC4

Additional XRCC4 Products

Product Documents for XRCC4 Antibody (OTI4H9) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for XRCC4 Antibody (OTI4H9) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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