YB1 Antibody (9J7M4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16213

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16213-100ul
NBP3-16213-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Chromatin Immunoprecipitation (ChIP), Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9J7M4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all YB1 Antibodies »

Product Summary for YB1 Antibody (9J7M4)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 225-324 of human YB1 (P67809). GAGEQGRPVRQNMYRGYRPRFRRGPPRQRQPREDGNEEDKENQGDETQGQQPPQRRYRRNFNYRRRRPENPKPQDGKETKAADPPAENSSAPEAEQGGAE

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for YB1 Antibody (9J7M4)

Western Blot: YB1 Antibody (9J7M4) [NBP3-16213]

Western Blot: YB1 Antibody (9J7M4) [NBP3-16213]

Western Blot: YB1 Antibody (9J7M4) [NBP3-16213] - Analysis of extracts of various cell lines, using YB1 Rabbit mAb (NBP3-16213) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: YB1 Antibody (9J7M4) [NBP3-16213]

Immunocytochemistry/ Immunofluorescence: YB1 Antibody (9J7M4) [NBP3-16213]

Immunocytochemistry/Immunofluorescence: YB1 Antibody (9J7M4) [NBP3-16213] - Immunofluorescence analysis of U-2 OS cells using YB1 Rabbit mAb (NBP3-16213) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: YB1 Antibody (9J7M4) [NBP3-16213]

Immunohistochemistry-Paraffin: YB1 Antibody (9J7M4) [NBP3-16213]

Immunohistochemistry-Paraffin: YB1 Antibody (9J7M4) [NBP3-16213] - Mouse brain using YB1 Rabbit mAb (NBP3-16213) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for YB1 Antibody (9J7M4)

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:500 - 1:1000

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: YB1

The Y-box binding protein 1 (YB1) is a pluripotent DNA/RNA-binding factor which regulates gene expression through transcription and translation. YB1 has been shown to be a marker of tumor aggressiveness and belongs to the cold-shock domain family.

Alternate Names

BP-8, CBF-A, class II, Y box-binding protein I, CSDA2, CSDB, DBPB CCAAT-binding transcription factor I subunit A, EFI-A, Enhancer factor I subunit A, MDR-NF1, MGC104858, MGC110976, MGC117250, NSEP1 Y-box-binding protein 1, nuclease-sensitive element-binding protein 1, Y box binding protein 1, YB1 DNA-binding protein B, YB-1 Y-box transcription factor, YBX1

Gene Symbol

YBX1

Additional YB1 Products

Product Documents for YB1 Antibody (9J7M4)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for YB1 Antibody (9J7M4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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