Ymer Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1542

Novus Biologicals, part of Bio-Techne
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NB100-1542
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Ymer Antibodies »

Product Specifications

Immunogen

Peptide with sequence CFSKSESSHKGFHYK corresponding to internal region according to NP_848018.1, NP_777568.1.

Epitope

internal region

Specificity

This antibody is expected to recognise both reported isoforms of human Ymer protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Ymer Antibody

Immunohistochemistry-Paraffin: Ymer Antibody [NB100-1542]

Immunohistochemistry-Paraffin: Ymer Antibody [NB100-1542]

Immunohistochemistry-Paraffin: Ymer Antibody [NB100-1542] - IHC analysis of Ymer in paraffin embedded Human Pancreas using NB100-1542 at 5ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for Ymer Antibody

Application
Recommended Usage

Immunohistochemistry

5 - 10 ug/ml

Immunohistochemistry-Paraffin

5 - 10 ug/ml

Peptide ELISA

Detection limit 1:32000
Application Notes
WB: Preliminary experiments gave an approx. 18 kDa band in human brain lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.3 kDa band according to NP_848018.1 and 35.8 kDa band according to NP_777568.1. The 18 kDa band was successfully blocked by incubation with the immunizing peptide. IHC-P: Human pancreas shows strong staining in cytoplasm in select acinar cells.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Ymer

Ymer encodes a soluble, cytoplasmic, tyrosine-phosphorylated protein with multiple ubiquitin-interacting domains. Mutations in this gene cause nonsyndromic, postlingual, progressive sensorineural DFNA44 hearing loss. In mouse, the protein is expressed in the inner ear during development and postnatal maturation and associates with microtubule-based structures. This protein may also function as a negative regulator of NF-kB signaling and as an effector of epidermal growth factor (EGF)-mediated cell signaling. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

Alternate Names

C3orf6deafness, autosomal dominant 44, coiled-coil domain containing 50, coiled-coil domain-containing protein 50, DFNA44, Protein Ymer, Ymer

Entrez Gene IDs

152137 (Human)

Gene Symbol

CCDC50

UniProt

NP_848018.1 (Human)

Additional Ymer Products

Product Documents for Ymer Antibody

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Certificate of Analysis

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Product Specific Notices for Ymer Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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