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Ymer Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88265

Novus Biologicals, part of Bio-Techne
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NBP1-88265
NBP1-88265-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QEKKDEDIARLLQEKELQEEKKRKKHFPEFPATRAYADSYYYEDGGMKPRVMKEAVSTPSRMAHRDQEWYDAEIARKLQEEELLATQVDMRAAQVAQDEEIARLLMAEEKKAYKKAKEREKSSLDKRKQDPEWKPKTAKAANSKSKESDE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ymer Antibody

Western Blot: Ymer Antibody [NBP1-88265]

Western Blot: Ymer Antibody [NBP1-88265]

Western Blot: Ymer Antibody [NBP1-88265] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: Ymer Antibody [NBP1-88265]

Immunocytochemistry/ Immunofluorescence: Ymer Antibody [NBP1-88265]

Immunocytochemistry/Immunofluorescence: Ymer Antibody [NBP1-88265] - Staining of human cell line U-251 MG shows localization to cytosol.
Immunohistochemistry-Paraffin: Ymer Antibody [NBP1-88265]

Immunohistochemistry-Paraffin: Ymer Antibody [NBP1-88265]

Immunohistochemistry-Paraffin: Ymer Antibody [NBP1-88265] - Staining of human lymph node shows strong cytoplasmic positivity in a subset of lymphoid cells outside reaction center.

Applications for Ymer Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ymer

Ymer encodes a soluble, cytoplasmic, tyrosine-phosphorylated protein with multiple ubiquitin-interacting domains. Mutations in this gene cause nonsyndromic, postlingual, progressive sensorineural DFNA44 hearing loss. In mouse, the protein is expressed in the inner ear during development and postnatal maturation and associates with microtubule-based structures. This protein may also function as a negative regulator of NF-kB signaling and as an effector of epidermal growth factor (EGF)-mediated cell signaling. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]

Alternate Names

C3orf6deafness, autosomal dominant 44, coiled-coil domain containing 50, coiled-coil domain-containing protein 50, DFNA44, Protein Ymer, Ymer

Gene Symbol

CCDC50

Additional Ymer Products

Product Documents for Ymer Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Ymer Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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