YOD1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84173

Novus Biologicals, part of Bio-Techne
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NBP1-84173
NBP1-84173-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Rat (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all YOD1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DMLIIEEDQTRPRSSPAFTKRGASSYVRETLPVLTRTVVPADNSCLFTSVYYVVEGGVLNPACAPEMRRLIAQIVA

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for YOD1 Antibody

Western Blot: YOD1 Antibody [NBP1-84173]

Western Blot: YOD1 Antibody [NBP1-84173]

Western Blot: YOD1 Antibody [NBP1-84173] - Analysis in control (vector only transfected HEK293T lysate) and YOD1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: YOD1 Antibody [NBP1-84173]

Immunocytochemistry/ Immunofluorescence: YOD1 Antibody [NBP1-84173]

Immunocytochemistry/Immunofluorescence: YOD1 Antibody [NBP1-84173] - Staining of human cell line A-431 shows localization to nucleoplasm, nuclear bodies & plasma membrane.
Immunohistochemistry-Paraffin: YOD1 Antibody [NBP1-84173]

Immunohistochemistry-Paraffin: YOD1 Antibody [NBP1-84173]

Immunohistochemistry-Paraffin: YOD1 Antibody [NBP1-84173] - Staining of human bone marrow shows cytoplasmic positivity in hematopoietic cells.

Applications for YOD1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: YOD1

Deubiquitinating enzymes (DUBs; see MIM 603478) are proteases that specifically cleave ubiquitin (MIM 191339) linkages, negating the action of ubiquitin ligases. DUBA8 belongs to a DUB subfamily characterized by an ovarian tumor (OTU) domain.(supplied by OMIM)

Long Name

YOD1 OTU Deubiquinating Enzyme 1 Homolog

Alternate Names

DUBA8, HIN-7, OTUD2

Gene Symbol

YOD1

Additional YOD1 Products

Product Documents for YOD1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for YOD1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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