YTHD1 Antibody (PSH0-23)

Western Blot: YTHD1 Antibody (PSH0-23) [NBP3-33109]

Western Blot: YTHD1 Antibody (PSH0-23) [NBP3-33109] - Western blot analysis of YTHD1 on different lysates with Rabbit anti-YTHD1 antibody (NBP3-33109) at 1/1,000 dilution.

Lane 1: HepG2 cell lysate (10 ug/Lane)
Lane 2: Hela cell lysate (10 ug/Lane)
Lane 3: 293T cell lysate (10 ug/Lane)
Lane 4: MCF-7 cell lysate (10 ug/Lane)
Lane 5: NIH/3T3 cell lysate (10 ug/Lane)
Lane 6: PC-12 cell lysate (10 ug/Lane)
Lane 7: HCT116 cell lysate (10 ug/Lane)
Lane 8: Mouse testis liver tissue lysate (20 ug/Lane)
Lane 9: Rat testis tissue lysate (20 ug/Lane)
Lane 10: Mouse brain tissue lysate (20 ug/Lane)
Lane 11: Rat brain tissue lysate (20 ug/Lane)

Predicted band size: 61 kDa
Observed band size: 69 kDa

Exposure time: 5 minutes;

10% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33109) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: YTHD1 Antibody (PSH0-23) [NBP3-33109]

Immunohistochemistry: YTHD1 Antibody (PSH0-23) [NBP3-33109] - Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-YTHD1 antibody (NBP3-33109) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33109) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: YTHD1 Antibody (PSH0-23) [NBP3-33109]

Flow Cytometry: YTHD1 Antibody (PSH0-23) [NBP3-33109] - Flow cytometric analysis of 293 cells labeling YTHD1.

Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-33109, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).