YTHDC1 Antibody (3K8D1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33486
Key Product Details
Species Reactivity
Human
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 3K8D1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 597-736 of mouse YTHDC1 (NP_808348.2).
Sequence:
SYNDYVREFHNMGPPPPWQGMPPYPGIEQPPHHPYYQHHAPPPQAHPPYSGHHPVPHEARYRDKRVHDYDMRVDDFLRRTQAVVSGRRSRPRERDRERERDRPRDNRRDRERDRGRDRERERERICDRDRDRGERGRYRR
Sequence:
SYNDYVREFHNMGPPPPWQGMPPYPGIEQPPHHPYYQHHAPPPQAHPPYSGHHPVPHEARYRDKRVHDYDMRVDDFLRRTQAVVSGRRSRPRERDRERERDRPRDNRRDRERDRGRDRERERERICDRDRDRGERGRYRR
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for YTHDC1 Antibody (3K8D1)
Western Blot: YTHDC1 Antibody (3K8D1) [NBP3-33486] -
Western Blot: YTHDC1 Antibody (3K8D1) [NBP3-33486] - Western Blot analysis of lysates from HeLa cells using [KO Validated] YTHDC1 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 45s.
Western Blot: YTHDC1 Antibody (3K8D1) [NBP3-33486] -
Western Blot: YTHDC1 Antibody (3K8D1) [NBP3-33486] - Western Blot analysis of lysates from YTHDC1-cKO mouse ES cells, using [KO Validated] YTHDC1 Rabbit mAb at 1:1000 dilution incubated overnight at 4C. YTHDC1-cKO mouse ES cells were treated by 4OHT.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 45s.
Applications for YTHDC1 Antibody (3K8D1)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 ug/mL
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: YTHDC1
Long Name
YTH Domain Containing 1
Alternate Names
KIAA1966, YT521, YT521-B
Additional YTHDC1 Products
Product Specific Notices for YTHDC1 Antibody (3K8D1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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