ZBTB4 Antibody

Western Blot: ZBTB4 Antibody [NBP3-18655]

Western Blot: ZBTB4 Antibody [NBP3-18655] - Figure 2. Western blot analysis using the antibody directed against ZBTB4 Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using the antibody against ZBTB4 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 105 kDa); the marker (in kDa) is shown on the left.

ELISA: ZBTB4 Antibody [NBP3-18655]

ELISA: ZBTB4 Antibody [NBP3-18655] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human ZBTB4 . The plates were coated with the peptides used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:50,000.