ZBTB46 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27211

Novus Biologicals, part of Bio-Techne
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NBP2-27211
NBP2-27211SS
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Chimpanzee (100%). Backed by our 100% Guarantee.

Applications

Validated:

ELISA

Cited:

ELISA

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all ZBTB46 Antibodies »

Product Specifications

Immunogen

Amino acids 62-87 of human RINZF protein were used as the immunogen.

Reactivity Notes

The NCBI accession number for ZBTB46 is Q96DT7. The amino acid sequence used as immunogen is 100% homologous in Chimpanzee and 88% homologous in Mouse.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZBTB46 Antibody

ELISA: ZBTB46 Antibody [NBP2-27211]

ELISA: ZBTB46 Antibody [NBP2-27211]

ELISA: ZBTB46 Antibody [NBP2-27211] - ELISA analysis of ZBTB10 expression. MDA-MB-231 cells were transiently transfected with 0-4 ug of a ZBTB10 expression plasmid and analyzed for ZBTB10 expression after 24 h. Columns, means for 3 replicate ELISAs. Bars, SE. *, P < 0.05.

Applications for ZBTB46 Antibody

Application
Recommended Usage

ELISA

1:100-1:1000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 ml PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZBTB46

RIN zinc finger protein (RINZF)/ZBTB10 is a novel zinc finger protein that inhibits Sp1-dependent activation. In this regard, ZBTB10 has been identified as a putative suppressor of Sp proteins such as Sp1, Sp3 and Sp4. Sp proteins belong to the Sp/kruppel-like factor (kLF) family of transcription factors and are overexpressed in certain cancers. Overexpression of Sp proteins is thought to contribute to the proliferative and angiogenic phenotype associated with cancer cells. Although the underlying factors associated with high expression of Sp proteins is not well understood, it is thought that microRNAs (miRNA) may play a role in mediating their overexpression in cancer cells. Like Sp proteins, miRNA-27a (miR-27a) is widely expressed in certain cancer cells. MiR-27a suppresses ZB1B10 expression and suppression of ZBTB10 by overexpressed miR-27a may be linked to overexpression of Sp proteins in breast cancer cells (Mertens-Talcott et al, 2007). These authors showed that introduction of antisense miR-27a resulted in increased expression of ZBTB10 mRNA and decreased expression of Sp1, Sp3 and Sp4 mRNA and protein. It is thought that the oncogenic activity of miR-27a may be due, in part, to suppression of ZBTB10 which leads to increased expression of Sp proteins thereby contributing to the proliferative and angiogenic cancer phenotype. Principal Names: Zinc finger and BTB domain containing 10 Official Gene Symbol: ZBTB10 Gene ID: 65986 (Human) Gene Map Locus: 8q13-q21.1 (Human) RINZF is a novel member of the Cys2-His2 zinc finger gene family, having a cDNA sequence of 3.8 kilobases. Apart from the Cys2-His2 zinc finger domains, the RIN ZF coding sequence bears little resemblance to other reported zinc finger genes. The amino-terminal region of the protein is an activation domain, despite the lack of recognizable activation motifs, such as homopolymeric glutamine and proline-rich stretches. Instead, it possesses several regions rich in basic residues, often associated with repressor domains. The gene encoding the RINZF protein is mapped on human chromosome 8q13-q21.1. The protein is highly conserved and acts as a transcriptional regulator. The protein competes with Sp1 binding at the CACC cis-regulatory element of the gastrin promoter and thus has a down-regulatory effect on gastrin transcription. The existence of two transcript variants, isoform 1 and 2, produced by alternate splicing of the RINZF gene has been studied.

Alternate Names

BTB (POZ) domain containing 4, BTB/POZ domain-containing protein 4, BTBD4, dJ583P15.7, dJ583P15.8, FLJ13502, RINZF, zinc finger and BTB domain containing 46, zinc finger and BTB domain-containing protein 46, Zinc finger protein 340, ZNF340

Gene Symbol

ZBTB46

Additional ZBTB46 Products

Product Documents for ZBTB46 Antibody

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Product Specific Notices for ZBTB46 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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