Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunoprecipitation, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ZBTB7A/Pokemon Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 200 and 250 of human ZBTB7 (Factor Binding IST protein 1) using the numbering given in entry NP_056982.1 (GeneID 51341).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZBTB7A/Pokemon Antibody

Western Blot: ZBTB7A/Pokemon Antibody [NB100-761]

Western Blot: ZBTB7A/Pokemon Antibody [NB100-761]

Western Blot: ZBTB7A/Pokemon Antibody [NB100-761] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZBTB7/FBI-1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: ZBTB7A/Pokemon Antibody [NB100-761]

Western Blot: ZBTB7A/Pokemon Antibody [NB100-761]

Western Blot: ZBTB7A/Pokemon Antibody [NB100-761] - Whole cell lysate (50 ug) from HeLa and HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ZBTB7/FBI-1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: ZBTB7A/Pokemon Antibody [NB100-761]

Immunoprecipitation: ZBTB7A/Pokemon Antibody [NB100-761]

Immunoprecipitation: ZBTB7A/Pokemon Antibody [NB100-761] - Detection of human ZBTB7/FBI-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZBTB7/FBI-1 antibody NB100-761 (lot NB100-761-3) used for IP at 3 ug per reaction. ZBTB7/FBI-1 was also immunoprecipitated by a previous lot of this antibody (lot NB100-761-2) and rabbit anti-ZBTB7/FBI-1 antibody NB100-762. For blotting immunoprecipitated ZBTB7/FBI-1, NB100-761 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for ZBTB7A/Pokemon Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:3000-1:15000
Application Notes
IHC reactivity reported in (PMID:20471975).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ZBTB7A/Pokemon

Pokemon (POK Erythroid Myeloid Ontogenic factor) is part of the POK gene family that encodes proteins. It is an oncogene, but its role is unique in that it controls the activity of other oncogenes. POK proteins are critical in embryonic development, cellular differentiation, and oncogenesis.

Long Name

Zinc Finger and BTB Domain-Containing Protein 7A

Alternate Names

FBI-1, LRF, Pokemon, TIP21

Entrez Gene IDs

51341 (Human); 16969 (Mouse)

Gene Symbol

ZBTB7A

UniProt

O95365

Additional ZBTB7A/Pokemon Products

Product Documents for ZBTB7A/Pokemon Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ZBTB7A/Pokemon Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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