ZC3H7A Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-06301
![Western Blot: ZC3H7A Antibody [NBP3-06301]](https://resources.bio-techne.com/images/products/ZC3H7A-Antibody-Western-Blot-NBP3-06301-img0002.jpg "Western Blot: ZC3H7A Antibody [NBP3-06301]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human ZC3H7A fragment.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for ZC3H7A Antibody
Western Blot: ZC3H7A Antibody [NBP3-06301]
Western Blot: ZC3H7A Antibody [NBP3-06301] - Anti-ZC3H7A rabbit polyclonal antibody at 1:500 dilutionLane A: Hela Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L) HRP at 110000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:110 kDa![Immunocytochemistry/ Immunofluorescence: ZC3H7A Antibody [NBP3-06301]](https://resources.bio-techne.com/images/products/ZC3H7A-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-06301-img0001.jpg "Immunocytochemistry/ Immunofluorescence: ZC3H7A Antibody [NBP3-06301]")
Immunocytochemistry/ Immunofluorescence: ZC3H7A Antibody [NBP3-06301]
Immunocytochemistry/Immunofluorescence: ZC3H7A Antibody [NBP3-06301] - Immunofluorescence staining of ZC3H7A in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human ZC3H7A polyclonal antibody (dilution ratio 1:200) at 4 degrees celsius overnight. Then cells were stained with the Alexa Fluor (TM) 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.![Immunoprecipitation: ZC3H7A Antibody [NBP3-06301]](https://resources.bio-techne.com/images/products/ZC3H7A-Antibody-Immunoprecipitation-NBP3-06301-img0003.jpg "Immunoprecipitation: ZC3H7A Antibody [NBP3-06301]")
Immunoprecipitation: ZC3H7A Antibody [NBP3-06301]
Immunoprecipitation: ZC3H7A Antibody [NBP3-06301] - ZC3H7A was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate4 uL anti-ZC3H7A rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein AG.Primary antibody:Anti-ZC3H7A rabbit polyclonal antibody, at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L) HRP at 110000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 110 kDaObserved band size :110 kDaApplications for ZC3H7A Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunoprecipitation
1-5ul/mg of lysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ZC3H7A
Alternate Names
FLJ10027, FLJ20318, HSPC055, ZC3H7, ZC3HDC7, zinc finger CCCH domain-containing protein 7A, zinc finger CCCH type domain containing 7, zinc finger CCCH-type containing 7, zinc finger CCCH-type containing 7A, zinc finger CCCH-type domain containing 7, zinc-finger protein AY163807
Gene Symbol
ZC3H7A
Additional ZC3H7A Products
Product Specific Notices for ZC3H7A Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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