ZCCHC11/TUT4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-783

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NB100-783
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all ZCCHC11/TUT4 Antibodies »

Product Specifications

Immunogen

Peptide with sequence RERCPHPPRGNVSE corresponding to C-Terminus according to NP_001009881.1, NP_056084.1, NP_001009882.1.

Specificity

This antibody is expected to recognize all 3 reported isoforms (as represented by NP_001009881.1; NP_056084.1; NP_001009882.1)

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ZCCHC11/TUT4 Antibody

Immunocytochemistry/ Immunofluorescence: ZCCHC11/TUT4 Antibody [NB100-783]

Immunocytochemistry/ Immunofluorescence: ZCCHC11/TUT4 Antibody [NB100-783]

Immunocytochemistry/Immunofluorescence: ZCCHC11/TUT4 Antibody [NB100-783] - Analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml)
Immunohistochemistry-Paraffin: ZCCHC11/TUT4 Antibody [NB100-783]

Immunohistochemistry-Paraffin: ZCCHC11/TUT4 Antibody [NB100-783]

Immunohistochemistry-Paraffin: ZCCHC11/TUT4 Antibody [NB100-783] - Staining of paraffin embedded Human Lymph Node. Antibody at 4 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Applications for ZCCHC11/TUT4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/ml

Immunohistochemistry

2 - 6 ug/mL

Immunohistochemistry-Paraffin

2 - 6 ug/mL

Peptide ELISA

Detection limit 1:16000
Application Notes
IHC-P: Human Lymph Node shows nuclear staining of select lymphocytes and staining of macrophages.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ZCCHC11/TUT4

Uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. Catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. Does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28 and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. May also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation

Alternate Names

DKFZp779C1943, EC 2.7.7.52, KIAA0191FLJ42878, PAP associated domain containing 3, PAPD3, terminal uridylyltransferase 4, TUT4, TUTase 4, Zinc finger CCHC domain-containing protein 11, zinc finger, CCHC domain containing 11

Entrez Gene IDs

23318 (Human)

Gene Symbol

TUT4

UniProt

Q5TAX3 (Human)

Additional ZCCHC11/TUT4 Products

Product Documents for ZCCHC11/TUT4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ZCCHC11/TUT4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.