ZCCHC11/TUT4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-27539

Novus Biologicals, part of Bio-Techne
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NBP2-27539
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Key Product Details

Species Reactivity

Mouse, Rat

Applications

Knockout Validated, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all ZCCHC11/TUT4 Antibodies »

Product Specifications

Immunogen

This ZCCHC11/TUT4 Antibody was developed against a peptide with sequence C-QKSAEKQGDLTPSET corresponding to internal region according to NP_780681.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for ZCCHC11/TUT4 Antibody

Western Blot: ZCCHC11/TUT4 Antibody [NBP2-27539]

Western Blot: ZCCHC11/TUT4 Antibody [NBP2-27539]

Western Blot: ZCCHC11/TUT4 Antibody [NBP2-27539] - Analysis of Rat Brain lysate (35 ug protein in RIPA buffer). Antibody at 0.05 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence. Approx. 170 kDa band observed in rat brain lysates (calculated MW of 163 kDa band according to rat NP_001101423.1).

Applications for ZCCHC11/TUT4 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

0.1 - 0.3 ug/mL

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ZCCHC11/TUT4

Uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. Catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. Does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28 and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. May also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation

Alternate Names

DKFZp779C1943, EC 2.7.7.52, KIAA0191FLJ42878, PAP associated domain containing 3, PAPD3, terminal uridylyltransferase 4, TUT4, TUTase 4, Zinc finger CCHC domain-containing protein 11, zinc finger, CCHC domain containing 11

Entrez Gene IDs

230594 (Mouse); 313481 (Rat)

Gene Symbol

TUT4

UniProt

NP_780681.2 (Human)

Additional ZCCHC11/TUT4 Products

Product Documents for ZCCHC11/TUT4 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ZCCHC11/TUT4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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