Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Knockdown Validated, Western Blot

Cited:

Immunoprecipitation, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ZCCHC8 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human zinc finger, CCHC domain containing 8 using the numbering given in entry NP_060082.2 (GeneID 55596).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZCCHC8 Antibody

Western Blot: ZCCHC8 Antibody [NB100-94995]

Western Blot: ZCCHC8 Antibody [NB100-94995]

Western Blot: ZCCHC8 Antibody [NB100-94995] - Analysis of ZCCHC8 in HeLa whole cell lsyate using anti-ZCCHC8 antibody. Lane1: Control siRNA , Lane 2: HeLa cells treated with ZCCHC8 siRNA . Image from verified customer review.
Immunohistochemistry-Paraffin: ZCCHC8 Antibody [NB100-94995]

Immunohistochemistry-Paraffin: ZCCHC8 Antibody [NB100-94995]

Immunohistochemistry-Paraffin: ZCCHC8 Antibody [NB100-94995] - Sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-used at a dilution of 1:5,000 (0.2ug/ml) and 1:2,000 (0.5ug/ml). Detection: DAB and Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain, cross-adsorbedAntibody DyLight (R) 594 Conjugated used at a dilution of 1:100
Immunoprecipitation: ZCCHC8 Antibody [NB100-94995]

Immunoprecipitation: ZCCHC8 Antibody [NB100-94995]

Immunoprecipitation: ZCCHC8 Antibody [NB100-94995] - Detection of Human ZCCHC8 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-ZCCHC8 antibody NB100-94995 used for IP at 3 mcg/mg lysate. ZCCHC8 was also immunoprecipitated by rabbit anti-ZCCHC8 antibody NB100-94996, which recognizes a downstream epitope.

Applications for ZCCHC8 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:1000 - 1:5000

Immunohistochemistry

1:2000 - 1:10000

Immunohistochemistry-Paraffin

1:2000 - 1:10000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:100-1:2000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. ZCCHC8 antibody validated for WB from a verified customer review.

Reviewed Applications

Read 2 reviews rated 5 using NB100-94995 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ZCCHC8

ZCCHC8 is a zinc-finger protein that bears a zinc-binding motif composed of a CX2CX4HX4C sequence (where X can be any amino acid). ZCCHC8 also bears a PSP domain, a proline rich domain found in numerous splicesome associated proteins. ZCCHC8 has been identified as a component of the splicesome C complex and as a substrate of glycogen synthase kinase-3. It is suggested to play a role in the processing and metabolism of RNA.

Alternate Names

DKFZp434E2220, zinc finger CCHC domain-containing protein 8, zinc finger, CCHC domain containing 8

Entrez Gene IDs

55596 (Human)

Gene Symbol

ZCCHC8

UniProt

NP_060082.2 (Human)

Additional ZCCHC8 Products

Product Documents for ZCCHC8 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ZCCHC8 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.