Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ZCRB1 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 155 to 205 of human Zinc Finger CCHC-type and RNA Binding Motif 1 using the numbering given in entry NP_149105.3 (GeneID 85437).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZCRB1 Antibody

Western Blot: ZCRB1 Antibody [NBP2-37757]

Western Blot: ZCRB1 Antibody [NBP2-37757]

Western Blot: ZCRB1 Antibody [NBP2-37757] - Detection of Human ZCRB1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZCRB1 antibody used for IP at 6 ug per reaction. ZCRB1 was also immunoprecipitated by rabbit anti-ZCRB1 antibody. For blotting immunoprecipitated ZCRB1, NBP2-37757 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: ZCRB1 Antibody [NBP2-37757]

Western Blot: ZCRB1 Antibody [NBP2-37757]

Western Blot: ZCRB1 Antibody [NBP2-37757] - Detection of Human and Mouse ZCRB1 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZCRB1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for ZCRB1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-20% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ZCRB1

Pre-mRNA splicing is catalyzed by the spliceosome. U12-type spliceosome binds U12-type pre-mRNAs and recognizes the 5' splice site and branch-point sequence. U11 and U12 snRNPs are components of U12-type spliceosome and function as a molecular bridge connecting both ends of the intron. The protein encoded by this gene contains a RNA recognition motif. It was identified as one of the protein components of U11/U12 snRNPs. This protein and many other U11/U12 snRNP proteins are highly conserved in organisms known to contain U12-type introns. These proteins have been shown to be essential for cell viability, suggesting the key roles in U12-type splicing. [provided by RefSeq]

Alternate Names

MADP-1, MADP1U11/U12 snRNP 31 kDa protein, MGC26805, RBM36, U11/U12 small nuclear ribonucleoprotein 31 kDa protein, U11/U12 snRNP 31K, U11/U12-31K, ZCCHC19, zinc finger CCHC-type and RNA binding motif 1, zinc finger CCHC-type and RNA-binding motif-containing protein 1

Gene Symbol

ZCRB1

UniProt

NP_149105.3 (Human)

Additional ZCRB1 Products

Product Documents for ZCRB1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ZCRB1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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