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ZDHHC9 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84499

Novus Biologicals, part of Bio-Techne
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NBP1-84499
NBP1-84499-25ul

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (91%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PSTQETSSSLLPQSPAPTEHLNSNEMPEDSSTPEEMPPPEPPEPPQEAAEAEK

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 26493479).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZDHHC9 Antibody

Western Blot: ZDHHC9 Antibody [NBP1-84499]

Western Blot: ZDHHC9 Antibody [NBP1-84499]

Western Blot: ZDHHC9 Antibody [NBP1-84499] - Analysis in human cell lines A-431 and U2OS. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PFN1.
Immunocytochemistry/ Immunofluorescence: ZDHHC9 Antibody [NBP1-84499]

Immunocytochemistry/ Immunofluorescence: ZDHHC9 Antibody [NBP1-84499]

Immunocytochemistry/Immunofluorescence: ZDHHC9 Antibody [NBP1-84499] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol & the Golgi apparatus.
Immunohistochemistry-Paraffin: ZDHHC9 Antibody [NBP1-84499]

Immunohistochemistry-Paraffin: ZDHHC9 Antibody [NBP1-84499]

Immunohistochemistry-Paraffin: ZDHHC9 Antibody [NBP1-84499] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.

Applications for ZDHHC9 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZDHHC9

The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS

Alternate Names

CGI89, CGI-89, chromosome X open reading frame 11, DHHC-9, EC 2.3.1, EC 2.3.1.-, EC 2.3.1.12, MRXSZ, ZDHHC10, Zinc finger DHHC domain-containing protein 9, zinc finger, DHHC-type containing 10, zinc finger, DHHC-type containing 9, ZNF379MMSA1, ZNF380DHHC domain containing 10

Gene Symbol

ZDHHC9

Additional ZDHHC9 Products

Product Documents for ZDHHC9 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ZDHHC9 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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