Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all ZEB1 Antibodies »

Product Specifications

Immunogen

Antibody was raised against a 15 amino acid synthetic peptide near the center of human ZEB1. The immunogen is located within amino acids 530 - 580 of ZEB1. Amino Acid Squence: HYDLKQPTQPPPLPA

Specificity

Multiple isoforms of ZEB1 are known to exist.

Marker

Mesenchymal Cells Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ZEB1 Antibody - BSA Free

Western Blot: ZEB1 AntibodyBSA Free [NBP1-77178]

Western Blot: ZEB1 AntibodyBSA Free [NBP1-77178]

Western Blot: ZEB1 Antibody [NBP1-77178] - HeLa cell lysate with ZEB1 antibody at 1ug/mL.
ZEB1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody - BSA Free [NBP1-77178] -

Immunocytochemistry/ Immunofluorescence: ZEB1 Antibody - BSA Free [NBP1-77178] - Immunocytochemistry of ZEB1 in K562 cells with ZEB1 antibody at 2.5 u/ml.
ZEB1 Antibody - BSA Free

Immunohistochemistry: ZEB1 Antibody - BSA Free [NBP1-77178] -

Immunohistochemistry: ZEB1 Antibody - BSA Free [NBP1-77178] - Immunohistochemistry of ZEB1 in human liver tissue with ZEB1 antibody at 5 u/mL.
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Applications for ZEB1 Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/mL

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1 ug/ml

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZEB1

ZEB1, initially identified as the delta-crystallin enhancer binding protein delta EF1, is a DNA-binding protein that binds to a modified E-box sequence and has been implicated in postgastrulation embryogenesis. ZEB1 binds to the promoter of several hemapoietic genes, including interleukin-2, CD4, GATA-3, and alpha(4)-integrin, and mice in which ZEB1 has been targeted show thymic atrophy, and severe defects in lymphocyte differentiation. Recent evidence suggests that ZEB1 also regulates the accumulation of adipose tissue and may play a role in obesity. Mutations in this gene have been associated with late-onset Fuchs endothelial corneal dystrophy.

Long Name

Zinc Finger E-box Binding Homeobox 1

Alternate Names

AREB6, BZP, DELTAEF1, FECD6, NIL2A, PPCD3, TCF8, ZFHEP

Gene Symbol

ZEB1

UniProt

CAI12550 (Human)

Additional ZEB1 Products

Product Documents for ZEB1 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ZEB1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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