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ZFPL1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85347

Novus Biologicals, part of Bio-Techne
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NBP1-85347
NBP1-85347-25ul

Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DEVVSPEPEPLNTSDFSDWSSFNASSTPGPEEVDSASAAPAFYSQAPRPPASPGRPEQHTVIHMGNPEPLTHAPRKVYDTRDDDRTPGLHGDCDDDKYRRRPALGWLARLLRSRAGSRKR

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (83%). Human reactivity reported in scientific literature (PMID: 25359980).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZFPL1 Antibody

Western Blot: ZFPL1 Antibody [NBP1-85347]

Western Blot: ZFPL1 Antibody [NBP1-85347]

Western Blot: ZFPL1 Antibody [NBP1-85347] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ZFPL1 antibody. Remaining relative intensity is presented.
Immunocytochemistry/ Immunofluorescence: ZFPL1 Antibody [NBP1-85347]

Immunocytochemistry/ Immunofluorescence: ZFPL1 Antibody [NBP1-85347]

Immunocytochemistry/Immunofluorescence: ZFPL1 Antibody [NBP1-85347] - Staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ZFPL1 Antibody [NBP1-85347]

Immunohistochemistry-Paraffin: ZFPL1 Antibody [NBP1-85347]

Immunohistochemistry-Paraffin: ZFPL1 Antibody [NBP1-85347] - Staining of human pancreas using Anti-ZFPL1 antibody NBP1-85347.

Applications for ZFPL1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF reported in scientific literature (PMID: 25359980). For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZFPL1

ZFPL1 is required for cis-Golgi integrity and efficient ER to Golgi transport. Involved in the maintainance of the integrity of the cis-Golgi, possibly via its interaction with GOLGA2/GM130

Alternate Names

D11S750, MCG4, Zinc finger protein MCG4, zinc finger protein-like 1, zinc-finger protein in MEN1 region

Gene Symbol

ZFPL1

Additional ZFPL1 Products

Product Documents for ZFPL1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ZFPL1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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