ZFYVE16 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36538
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by the antibody maps to a region between residue 450 to 500 of human Endosome-Associated FYVE Domain Protein using the numbering given in entry NP_001098721.1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ZFYVE16 Antibody
Western Blot: ZFYVE16 Antibody [NBP2-36538]
Western Blot: ZFYVE16 Antibody [NBP2-36538] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Endofin antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.Immunoprecipitation: ZFYVE16 Antibody [NBP2-36538]
Immunoprecipitation: ZFYVE16 Antibody [NBP2-36538] - Detection of human Endofin by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Endofin antibody NBP2-36538) used for IP at 6 ug per reaction. Endofin was also immunoprecipitated by rabbit anti-Endofin antibody NBP2-36539. For blotting immunoprecipitated Endofin, NBP2-36538 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.Applications for ZFYVE16 Antibody
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg
Western Blot
1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.
Validation by IP/Western Blot was performed using a 4-8% SDS-PAGE gel.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ZFYVE16
Alternate Names
DKFZp686E13162, Endofin, endosome-associated FYVE-domain protein, KIAA0305Endosome-associated FYVE domain protein, zinc finger FYVE domain-containing protein 16, zinc finger, FYVE domain containing 16
Gene Symbol
ZFYVE16
UniProt
Additional ZFYVE16 Products
Product Documents for ZFYVE16 Antibody
Product Specific Notices for ZFYVE16 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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