Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Primate (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ZHX1 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residues 1-50 of human zinc fingers and homeoboxes 1 using the TrEMBL entry Q9UKY1 (GeneID 11244).

Reactivity Notes

Chimpanzee (100%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZHX1 Antibody

Western Blot: ZHX1 Antibody [NB600-244]

Western Blot: ZHX1 Antibody [NB600-244]

Western Blot: ZHX1 Antibody [NB600-244] - Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbitanti-ZHX1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: ZHX1 Antibody [NB600-244]

Immunohistochemistry-Paraffin: ZHX1 Antibody [NB600-244]

Immunohistochemistry-Paraffin: ZHX1 Antibody [NB600-244] - Section of human lung carcinoma. Antibody: Affinity purified rabbit anti- ZHX1 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: ZHX1 Antibody [NB600-244]

Immunoprecipitation: ZHX1 Antibody [NB600-244]

Immunoprecipitation: ZHX1 Antibody [NB600-244] - Detection of human ZHX1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ZHX1 antibody NB600-244 used for IP at 3 ug per reaction. ZHX1 was also immunoprecipitated by another rabbit anti-ZHX1 antibody. For blotting immunoprecipitated ZHX1, NB600-244 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.

Applications for ZHX1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ZHX1

Zinc-fingers and homeoboxes 1 (ZHX1) is a protein which interacts with the activation domain of the A subunit of nuclear factor-Y. It is a transcriptional repressor protein.

Alternate Names

zona pellucida B protein, zona pellucida glycoprotein 4ZBP, Zona pellucida protein B, zona pellucida sperm-binding protein 4, Zp-4, ZPBZP1

Entrez Gene IDs

11244 (Human)

Gene Symbol

ZHX1

UniProt

Q9UKY1 (Human)

Additional ZHX1 Products

Product Documents for ZHX1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ZHX1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.