ZIC4 Antibody (4B1)
Novus Biologicals, part of Bio-Techne | Catalog # H00084107-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 4B1
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
ZIC4 (NP_115529, 249 a.a. ~ 319 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SDRKKHSHVHTSDKPYTCKVRGCDKCYTHPSSLRKHMKVHGRSPPPSSGYDSATPSALVSPSSDCGHKSQV
Reactivity Notes
Human. Other species not tested.
Specificity
ZIC4 - Zic family member 4
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for ZIC4 Antibody (4B1)
Western Blot: ZIC4 Antibody (4B1) [H00084107-M01]
Western Blot: ZIC4 Antibody (4B1) [H00084107-M01] - ZIC4 monoclonal antibody (M01), clone 4B1 Analysis of ZIC4 expression in Hela S3 NE.Immunocytochemistry/ Immunofluorescence: ZIC4 Antibody (4B1) [H00084107-M01]
Immunocytochemistry/Immunofluorescence: ZIC4 Antibody (4B1) [H00084107-M01] - Analysis of monoclonal antibody to ZIC4 on HeLa cell. Antibody concentration 25 ug/ml.
Sandwich ELISA: ZIC4 Antibody (4B1) [H00084107-M01] - Detection limit for recombinant GST tagged ZIC4 is approximately 0.03ng/ml as a capture antibody.
Applications for ZIC4 Antibody (4B1)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: ZIC4
Alternate Names
FLJ42609, FLJ45833, Zic family member 4, Zinc finger protein of the cerebellum 4zinc family member 4 protein HZIC4, zinc finger protein ZIC 4
Entrez Gene IDs
84107 (Human)
Gene Symbol
ZIC4
OMIM
608948 (Human)
UniProt
Additional ZIC4 Products
Product Documents for ZIC4 Antibody (4B1)
Product Specific Notices for ZIC4 Antibody (4B1)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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