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ZIC4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37867

Novus Biologicals, part of Bio-Techne
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NBP2-37867
NBP2-37867-25ul

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LVMRKRLRLYRNTLKESSSSSGHHGPQLTAASSPSVFPGLHEEPPQASPSRPLNGLLRLGLPGDMYARPEPFPPGPAARSDALAAAAALHGYGGMNLTVNL

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (85%), Rat (84%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZIC4 Antibody

Immunocytochemistry/ Immunofluorescence: ZIC4 Antibody [NBP2-37867]

Immunocytochemistry/ Immunofluorescence: ZIC4 Antibody [NBP2-37867]

Immunocytochemistry/Immunofluorescence: ZIC4 Antibody [NBP2-37867] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleus & nucleoli.
Immunohistochemistry-Paraffin: ZIC4 Antibody [NBP2-37867]

Immunohistochemistry-Paraffin: ZIC4 Antibody [NBP2-37867]

Immunohistochemistry-Paraffin: ZIC4 Antibody [NBP2-37867] - Staining of human cerebral cortex shows nucleolar positivity in neuronal cells.
ZIC4 Antibody

Immunohistochemistry-Paraffin: ZIC4 Antibody [NBP2-37867] -

Immunohistochemistry-Paraffin: ZIC4 Antibody [NBP2-37867] - Staining of human cerebellum shows moderate nuclear positivity in neurons.

Applications for ZIC4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZIC4

ZIC4 encodes a member of the ZIC family of C2H2-type zinc finger proteins. Members of this family are important during development, and have been associated with X-linked visceral heterotaxy and holoprosencephaly type 5. This gene is closely linked to the gene encoding zinc finger protein of the cerebellum 1, a related family member on chromosome 3. This gene encodes a protein of unknown function.

Alternate Names

FLJ42609, FLJ45833, Zic family member 4, Zinc finger protein of the cerebellum 4zinc family member 4 protein HZIC4, zinc finger protein ZIC 4

Gene Symbol

ZIC4

UniProt

Additional ZIC4 Products

Product Documents for ZIC4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ZIC4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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