ZIM2 Antibody (OTI7G1) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-74929

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI7G1

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all ZIM2 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 1-150 and 428-527 of human ZIM2 (NP_056178) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ZIM2 Antibody (OTI7G1) - Azide and BSA Free

Western Blot: ZIM2 Antibody (OTI7G1)Azide and BSA Free [NBP2-74929]

Western Blot: ZIM2 Antibody (OTI7G1)Azide and BSA Free [NBP2-74929]

Western Blot: ZIM2 Antibody (OTI7G1) - Azide and BSA Free [NBP2-74929] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZIM2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZIM2.
Immunocytochemistry/ Immunofluorescence: ZIM2 Antibody (OTI7G1) - Azide and BSA Free [NBP2-74929]

Immunocytochemistry/ Immunofluorescence: ZIM2 Antibody (OTI7G1) - Azide and BSA Free [NBP2-74929]

Immunocytochemistry/Immunofluorescence: ZIM2 Antibody (OTI7G1) - Azide and BSA Free [NBP2-74929] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY ZIM2.
Immunohistochemistry: ZIM2 Antibody (OTI7G1) - Azide and BSA Free [NBP2-74929]

Immunohistochemistry: ZIM2 Antibody (OTI7G1) - Azide and BSA Free [NBP2-74929]

Immunohistochemistry: ZIM2 Antibody (OTI7G1) - Azide and BSA Free [NBP2-74929] - Staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-ZIM2 mouse monoclonal antibody.
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Applications for ZIM2 Antibody (OTI7G1) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ZIM2

In human, ZIM2 and PEG3 (GeneID:5178) are two distinct genes that share a set of 5' exons and have a common promoter, and both genes are paternally expressed (PMID:15203203). Alternative splicing events connect the shared exons either with the remaining 4 exons unique to ZIM2, or with the remaining 2 exons unique to PEG3. This is in contrast to mouse and cow, where ZIM2 and PEG3 genes do not share exons in common, and the imprinting status of ZIM2 is also not conserved amongst mammals. Additional 5' alternatively spliced transcripts encoding the same protein have been found for the human ZIM2 gene. (provided by RefSeq)

Alternate Names

zinc finger imprinted 2, zinc finger, imprinted 2, ZNF656Zinc finger protein 656

Gene Symbol

ZIM2

Additional ZIM2 Products

Product Documents for ZIM2 Antibody (OTI7G1) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZIM2 Antibody (OTI7G1) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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