ZNF143 Antibody

Western Blot: ZNF143 Antibody [NBP3-12663]

Western Blot: ZNF143 Antibody [NBP3-12663] - Lane A: HeLa Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: 293T Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:69 kDaObserved band size:72 kDa(We are unsure as to the identity of these extra bands.)

Immunocytochemistry/ Immunofluorescence: ZNF143 Antibody [NBP3-12663]

Immunocytochemistry/Immunofluorescence: ZNF143 Antibody [NBP3-12663] - Staining of ZNF143 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human ZNF143 polyclonal antibody (dilution ratio 1:1000) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus and Cytoplasm.

Immunoprecipitation: ZNF143 Antibody [NBP3-12663]

Immunoprecipitation: ZNF143 Antibody [NBP3-12663] - Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-ZNF143 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-ZNF143 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 69 kDaObserved band size :70 kDa