ZNF174 Antibody (2D7-E9)
Novus Biologicals, part of Bio-Techne | Catalog # H00007727-M01
![Western Blot: ZNF174 Antibody (2D7-E9) [H00007727-M01]](https://resources.bio-techne.com/images/products/ZNF174-Antibody-3E4-Western-Blot-H00007727-M01-img0012.jpg "Western Blot: ZNF174 Antibody (2D7-E9) [H00007727-M01]")
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # 2D7-E9
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
ZNF174 (AAH00876, 1 a.a. ~ 234 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAAKMEITLSSNTEASSKQERHIIAKLEEKRGPPLQKNCPDPELCRQSFRRFCYQEVSGPQEALSQLRQLCRQWLQPELHTKEQILELLVMEQFLTILPPEIQARVRHRCPMSSKEIVTLVEDFHRASKKPKQWVAVCMQGQKVLLEKTGSQLGEQELPDFQPQTPRRDLRESSPAEPSQAGAYDRLSPHHWEKSPLLQEPTPKLAGTELLIEKTDPNMATDELPCKLWLSFIA
Localization
Nuclear
Specificity
ZNF174 - zinc finger protein 174
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Scientific Data Images for ZNF174 Antibody (2D7-E9)
![Western Blot: ZNF174 Antibody (2D7-E9) [H00007727-M01]](https://resources.bio-techne.com/images/products/ZNF174-Antibody-3E4-Western-Blot-H00007727-M01-img0011.jpg "Western Blot: ZNF174 Antibody (2D7-E9) [H00007727-M01]")
Western Blot: ZNF174 Antibody (2D7-E9) [H00007727-M01]
Western Blot: ZNF174 Antibody (3E4) [H00007727-M01] - ZNF174 monoclonal antibody (M01), clone 2D7-E9 Analysis of ZNF174 expression in SW-13.![Immunocytochemistry/ Immunofluorescence: ZNF174 Antibody (2D7-E9) [H00007727-M01]](https://resources.bio-techne.com/images/products/ZNF174-Antibody-3E4-Immunocytochemistry-Immunofluorescence-H00007727-M01-img0008.jpg "Immunocytochemistry/ Immunofluorescence: ZNF174 Antibody (2D7-E9) [H00007727-M01]")
Immunocytochemistry/ Immunofluorescence: ZNF174 Antibody (2D7-E9) [H00007727-M01]
Immunocytochemistry/Immunofluorescence: ZNF174 Antibody (3E4) [H00007727-M01] - Analysis of monoclonal antibody to ZNF174 on HeLa cell. Antibody concentration 10 ug/ml.![Western Blot: ZNF174 Antibody (2D7-E9) [H00007727-M01]](https://resources.bio-techne.com/images/products/ZNF174-Antibody-3E4-Western-Blot-H00007727-M01-img0009.jpg "Western Blot: ZNF174 Antibody (2D7-E9) [H00007727-M01]")
Western Blot: ZNF174 Antibody (2D7-E9) [H00007727-M01]
Western Blot: ZNF174 Antibody (3E4) [H00007727-M01] - Analysis of ZNF174 over-expressed 293 cell line, cotransfected with ZNF174 Validated Chimera RNAi ( Cat # H00007727-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZNF174 monoclonal antibody (M01), clone 2D7-E9 (Cat # H00007727-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Applications for ZNF174 Antibody (2D7-E9)
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Knockdown Validated
Optimal dilutions of this antibody should be experimentally determined.
Sandwich ELISA
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF, RNAi Validation and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: ZNF174
Alternate Names
AW-1, zinc finger protein 174, ZSCAN8Zinc finger and SCAN domain-containing protein 8
Entrez Gene IDs
7727 (Human)
Gene Symbol
ZNF174
OMIM
603900 (Human)
UniProt
Additional ZNF174 Products
Product Documents for ZNF174 Antibody (2D7-E9)
Product Specific Notices for ZNF174 Antibody (2D7-E9)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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