ZNF277 Antibody (1B2)
Novus Biologicals, part of Bio-Techne | Catalog # H00011179-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # 1B2
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
ZNF277 (AAH20626, 1 a.a. ~ 278 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MQEDRDGSCSTVGGVGYGDSKDCILEPLSLPESPGGTTTLEGSPSVPCIFCEEHFPVAEQDKLLKHMIIEHKIVIADVKLVADFQRYILYWRKRFTEQPITDFCSVIRINSTAPFEEQENYFLLCDVLPEDRILREELQKQRLREILEQQQQERNDTNFHGVCMFCNEEFLGNRSVILNHMAREHAFNIGLPDNIVNCNEFLCTLQKKLDNLQCLYCEKTFRDKNTLKDHMRKKQHRKINPKNREYDRFYVINYLVRLSFHNLKFDCSTANKIFLRTN
Reactivity Notes
Human. Other species not tested.
Localization
Nuclear
Specificity
ZNF277 - zinc finger protein 277
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Scientific Data Images for ZNF277 Antibody (1B2)
![Western Blot: ZNF277 Antibody (1B2) [H00011179-M01]](https://resources.bio-techne.com/images/products/ZNF277-Antibody-1B2-Western-Blot-H00011179-M01-img0005.jpg "Western Blot: ZNF277 Antibody (1B2) [H00011179-M01]")
Western Blot: ZNF277 Antibody (1B2) [H00011179-M01]
Western Blot: ZNF277 Antibody (1B2) [H00011179-M01] - ZNF277 monoclonal antibody (M01), clone 1B2 Analysis of ZNF277 expression in Jurkat.![Western Blot: ZNF277 Antibody (1B2) [H00011179-M01]](https://resources.bio-techne.com/images/products/ZNF277-Antibody-1B2-Western-Blot-H00011179-M01-img0004.jpg "Western Blot: ZNF277 Antibody (1B2) [H00011179-M01]")
Western Blot: ZNF277 Antibody (1B2) [H00011179-M01]
Western Blot: ZNF277 Antibody (1B2) [H00011179-M01] - Analysis of ZNF277 over-expressed 293 cell line, cotransfected with ZNF277 Validated Chimera RNAi ( Cat # H00011179-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with ZNF277 monoclonal antibody (M01), clone 1B2 (Cat # H00011179-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.![Western Blot: ZNF277 Antibody (1B2) [H00011179-M01]](https://resources.bio-techne.com/images/products/ZNF277-Antibody-1B2-Western-Blot-H00011179-M01-img0006.jpg "Western Blot: ZNF277 Antibody (1B2) [H00011179-M01]")
Western Blot: ZNF277 Antibody (1B2) [H00011179-M01]
Western Blot: ZNF277 Antibody (1B2) [H00011179-M01] - Analysis of ZNF277 expression in transfected 293T cell line by ZNF277 monoclonal antibody (M01), clone 1B2.Lane 1: ZNF277 transfected lysate(51.6 KDa).Lane 2: Non-transfected lysate.Applications for ZNF277 Antibody (1B2)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for RNAi validation and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: ZNF277
Alternate Names
FLJ40981, zinc finger protein 433
Entrez Gene IDs
11179 (Human)
Gene Symbol
ZNF277
OMIM
605465 (Human)
UniProt
Additional ZNF277 Products
Product Documents for ZNF277 Antibody (1B2)
Product Specific Notices for ZNF277 Antibody (1B2)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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