Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ZNF295 Antibodies »

Product Summary for ZNF295 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 875 and 925 of human zinc finger protein 295 using the numbering given in entry NP_065778.3 (GeneID 49854).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZNF295 Antibody

Western Blot: ZNF295 Antibody [NB100-74655]

Western Blot: ZNF295 Antibody [NB100-74655]

Western Blot: ZNF295 Antibody [NB100-74655] - Detection of Human ZNF295 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-ZNF295 antibody NB100-74655 used for IP at 3 mcg/mg lysate. ZNF295 was also immunoprecipitated by rabbit anti-ZNF295 antibody NB100-74656, which recognizes a downstream epitope.
Immunocytochemistry/ Immunofluorescence: ZNF295 Antibody [NB100-74655]

Immunocytochemistry/ Immunofluorescence: ZNF295 Antibody [NB100-74655]

Immunocytochemistry/Immunofluorescence: ZNF295 Antibody [NB100-74655] - Detection: DAB and Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain, cross-adsorbed Antibody DyLight 594 Conjugated used at a dilution of 1:100
Immunohistochemistry: ZNF295 Antibody [NB100-74655]

Immunohistochemistry: ZNF295 Antibody [NB100-74655]

Immunohistochemistry: ZNF295 Antibody [NB100-74655] - Sample: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-ZNF295 used at a dilution of 1:1,000 (1ug/ml) and 1:400 (2.5ug/ml).

Applications for ZNF295 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:250-1:1000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

2-5ug/mg Lysate
Application Notes
This antibody is not recommended for Western Blot analysis.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ZNF295

ZNF295 bears 8 C2H2-type zinc fingers and a BTB/POZ (broad-complex, tramtrack, bric a brac/ Pox-virus and zinc finger) domain indicating a function in transcriptional regulation. ZNF295 has been shown to function as a transcriptional repressor and associates with ZFP161, a zinc-finger protein also know to function as a repressor of transcription.

Alternate Names

MGC15716, ZFP414, zinc finger protein 414

Entrez Gene IDs

49854 (Human)

Gene Symbol

ZNF295

UniProt

NP_065778.3 (Human)

Additional ZNF295 Products

Product Documents for ZNF295 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ZNF295 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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