ZNF295 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-82923

Novus Biologicals, part of Bio-Techne
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NBP1-82923
NBP1-82923-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ZNF295 Antibodies »

Product Summary for ZNF295 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QPEPNKVNHIVTTKDDNVFSDSSEQVNFDSEDSSCLPEDLSLSKQLKIQVKEEPVEEAEEEAPEASTAPKEAGPSKEASLWPCEKCGKMFTVHKQLERHQELLCSVKPFICHVCNKAFRTNFRLWSHFQSHMSQASEESAHKESEVCP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZNF295 Antibody

Western Blot: ZNF295 Antibody [NBP1-82923]

Western Blot: ZNF295 Antibody [NBP1-82923]

Western Blot: ZNF295 Antibody [NBP1-82923] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-660
Immunocytochemistry/ Immunofluorescence: ZNF295 Antibody [NBP1-82923]

Immunocytochemistry/ Immunofluorescence: ZNF295 Antibody [NBP1-82923]

Immunocytochemistry/Immunofluorescence: ZNF295 Antibody [NBP1-82923] - Staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: ZNF295 Antibody [NBP1-82923]

Immunohistochemistry-Paraffin: ZNF295 Antibody [NBP1-82923]

Immunohistochemistry-Paraffin: ZNF295 Antibody [NBP1-82923] - Staining in human adrenal gland and skeletal muscle tissues using anti-ZBTB21 antibody. Corresponding ZBTB21 RNA-seq data are presented for the same tissues.
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Applications for ZNF295 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZNF295

ZNF295 bears 8 C2H2-type zinc fingers and a BTB/POZ (broad-complex, tramtrack, bric a brac/ Pox-virus and zinc finger) domain indicating a function in transcriptional regulation. ZNF295 has been shown to function as a transcriptional repressor and associates with ZFP161, a zinc-finger protein also know to function as a repressor of transcription.

Alternate Names

MGC15716, ZFP414, zinc finger protein 414

Gene Symbol

ZNF295

Additional ZNF295 Products

Product Documents for ZNF295 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZNF295 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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