ZNF364 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-85586

Novus Biologicals, part of Bio-Techne
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NBP1-85586
NBP1-85586-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (90%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ZNF364 Antibodies »

Product Summary for ZNF364 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: MAEASAAGADSGAAVAAHRFFCHFCKGEVSPKLPEYICPRCESGFIEEVTDDSSFLGGGGSRIDNTTTTHFAELWGHLDHTMFFQDFRPFLSSSPLDQDNRAN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZNF364 Antibody

Western Blot: ZNF364 Antibody [NBP1-85586]

Western Blot: ZNF364 Antibody [NBP1-85586]

Western Blot: ZNF364 Antibody [NBP1-85586] - Analysis in control (vector only transfected HEK293T lysate) and rNF115 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: ZNF364 Antibody [NBP1-85586]

Immunocytochemistry/ Immunofluorescence: ZNF364 Antibody [NBP1-85586]

Immunocytochemistry/Immunofluorescence: ZNF364 Antibody [NBP1-85586] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli & mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ZNF364 Antibody [NBP1-85586]

Immunohistochemistry-Paraffin: ZNF364 Antibody [NBP1-85586]

Immunohistochemistry-Paraffin: ZNF364 Antibody [NBP1-85586] - Staining of human stomach shows strong cytoplasmic positivity in chief cells.

Applications for ZNF364 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZNF364

The RING finger family of proteins possess ubiquitin ligase activity and play pivotal roles in protein degradation and receptor-mediated endocytosis. The autoubiquitination activity of ZNF364 indicates that it is a novel RING-type E3 ligase. Its effects on cell growth indicate that ZNF364 may be important for the ubiquitin modification of proteins crucial to breast carcinogenesis and growth.

Alternate Names

E3 ubiquitin-protein ligase RNF115, EC 6.3.2.-, Rabring 7, ring finger protein 115CL469780, Zinc finger protein 364BCA2, ZNF364

Gene Symbol

RNF115

Additional ZNF364 Products

Product Documents for ZNF364 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZNF364 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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