ZNF521 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84883

Novus Biologicals, part of Bio-Techne
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NBP1-84883
NBP1-84883-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ZNF521 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KQDLVKLDINGLPYGLCAGCVNLSKSASPGINVPPGTNRPGLGQNENLSAIEGKGKVGGLKTRCSSCNVKFESE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (85%). Mouse reactivity reported in scientific literature (PMID: 23907569).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZNF521 Antibody

Immunohistochemistry-Paraffin: ZNF521 Antibody [NBP1-84883]

Immunohistochemistry-Paraffin: ZNF521 Antibody [NBP1-84883]

Immunohistochemistry-Paraffin: ZNF521 Antibody [NBP1-84883] - Staining of human colon, kidney, testis and vagina using Anti-ZNF521 antibody NBP1-84883 (A) shows similar protein distribution across tissues to independent antibody NBP1-84882 (B).
Immunocytochemistry/ Immunofluorescence: ZNF521 Antibody [NBP1-84883]

Immunocytochemistry/ Immunofluorescence: ZNF521 Antibody [NBP1-84883]

Immunocytochemistry/Immunofluorescence: ZNF521 Antibody [NBP1-84883] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
Immunohistochemistry-Paraffin: ZNF521 Antibody [NBP1-84883]

Immunohistochemistry-Paraffin: ZNF521 Antibody [NBP1-84883]

Immunohistochemistry-Paraffin: ZNF521 Antibody [NBP1-84883] - Staining of human placenta shows moderate to strong nuclear positivity in trophoblastic cells.
View 3 more images

Applications for ZNF521 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZNF521

Transcription factor that can both act as an activator or a repressor depending on the context. Involved in BMP signaling and in the regulation of the immature compartment of the hematopoietic system. Associates with SMADs in response to BMP2 leading to activate transcription of BMP target genes. Acts as a transcriptional repressor via its interaction with EBF1, a transcription factor involved specification of B-cell lineage; this interaction preventing EBF1 to bind DNA and activate target genes

Alternate Names

EHZF, Evi3, LIP3, ZFP521, zinc finger protein 263, Zinc finger protein FPM315, Zinc finger protein with KRAB and SCAN domains 12

Gene Symbol

ZNF521

Additional ZNF521 Products

Product Documents for ZNF521 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZNF521 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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