ZNF548 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-39065
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for ZNF548 Antibody
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: HRVHMAEEIFTCMEGWKDLPATSCLLQHQGPQSEWKPYRDTEDREAFQTGQ
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for ZNF548 Antibody
![Western Blot: ZNF548 Antibody [NBP2-39065]](https://resources.bio-techne.com/images/products/ZNF548-Antibody-Western-Blot-NBP2-39065-img0002.jpg "Western Blot: ZNF548 Antibody [NBP2-39065]")
Western Blot: ZNF548 Antibody [NBP2-39065]
Western Blot: ZNF548 Antibody [NBP2-39065] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG![Immunocytochemistry/ Immunofluorescence: ZNF548 Antibody [NBP2-39065]](https://resources.bio-techne.com/images/products/ZNF548-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-39065-img0003.jpg "Immunocytochemistry/ Immunofluorescence: ZNF548 Antibody [NBP2-39065]")
Immunocytochemistry/ Immunofluorescence: ZNF548 Antibody [NBP2-39065]
Immunocytochemistry/Immunofluorescence: ZNF548 Antibody [NBP2-39065] - Staining of human cell line HeLa shows localization to nucleoplasm & cytosol.![Immunohistochemistry: ZNF548 Antibody [NBP2-39065]](https://resources.bio-techne.com/images/products/ZNF548-Antibody-Immunohistochemistry-NBP2-39065-img0001.jpg "Immunohistochemistry: ZNF548 Antibody [NBP2-39065]")
Immunohistochemistry: ZNF548 Antibody [NBP2-39065]
Immunohistochemistry: ZNF548 Antibody [NBP2-39065] - Staining of human stomach shows strong cytoplasmic positivity in glandular cells.Applications for ZNF548 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: ZNF548
Additional ZNF548 Products
Product Documents for ZNF548 Antibody
Product Specific Notices for ZNF548 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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