ZNF650 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87996

Novus Biologicals, part of Bio-Techne
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NBP1-87996
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ZNF650 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DRSAWKHAGALKKSTCDAEKSYEVLLSFVISELFKGKLYHEEGTQECAMVNPIAWSPESMEKCLQDFCLPFLRITSLLQHHLFGEDLPSCQE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ZNF650 Antibody

Western Blot: ZNF650 Antibody [NBP1-87996]

Western Blot: ZNF650 Antibody [NBP1-87996]

Western Blot: ZNF650 Antibody [NBP1-87996] - Analysis in human cell line K562.
Immunocytochemistry/ Immunofluorescence: ZNF650 Antibody [NBP1-87996]

Immunocytochemistry/ Immunofluorescence: ZNF650 Antibody [NBP1-87996]

Immunocytochemistry/Immunofluorescence: ZNF650 Antibody [NBP1-87996] - Staining of human cell line A-431 shows localization to nucleus & nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: ZNF650 Antibody [NBP1-87996]

Immunohistochemistry-Paraffin: ZNF650 Antibody [NBP1-87996]

Immunohistochemistry-Paraffin: ZNF650 Antibody [NBP1-87996] - Staining of human liver shows moderate membranous and cytoplasmic positivity in hepatocytes.
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Applications for ZNF650 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ZNF650

E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Does not bind to proteins bearingspecific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination andsubsequent degradation

Alternate Names

DKFZp434P117, E3 ubiquitin-protein ligase UBR3, EC 6.3.2.-, FLJ37422, KIAA2024DKFZp686N10185, MGC126489, N-recognin-3, ubiquitin protein ligase E3 component n-recognin 3 (putative), Ubiquitin-protein ligase E3-alpha-3, Ubiquitin-protein ligase E3-alpha-III, Zinc finger protein 650ZNF650

Gene Symbol

UBR3

Additional ZNF650 Products

Product Documents for ZNF650 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ZNF650 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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