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ZNFN1A4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12160

Novus Biologicals, part of Bio-Techne
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NBP3-12160

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Peptide with sequence C-RPTFIDRLANSLTKR corresponding to internal region according to NP_071910.3.

Clonality

Polyclonal

Host

Goat

Scientific Data Images for ZNFN1A4 Antibody

Western Blot: ZNFN1A4 Antibody [NBP3-12160]

Western Blot: ZNFN1A4 Antibody [NBP3-12160]

Western Blot: ZNFN1A4 Antibody [NBP3-12160] - Staining of Jurkat nuclear lysate (A) + Peptide (B) with antibody at 2 ug/mL, and staining of K562 nuclear lysate (C) + Peptide (D) with antibody at 0.1 ug/mL, (35 ug protein in RIPA buffer). Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: ZNFN1A4 Antibody [NBP3-12160]

Immunocytochemistry/ Immunofluorescence: ZNFN1A4 Antibody [NBP3-12160]

Immunocytochemistry/Immunofluorescence: ZNFN1A4 Antibody [NBP3-12160] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry: ZNFN1A4 Antibody [NBP3-12160]

Flow Cytometry: ZNFN1A4 Antibody [NBP3-12160]

Flow Cytometry: ZNFN1A4 Antibody [NBP3-12160] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Applications for ZNFN1A4 Antibody

Application
Recommended Usage

Flow Cytometry

10 ug/mL

Immunocytochemistry/ Immunofluorescence

5 ug/mL

Peptide ELISA

Detection limit 1:2000

Western Blot

0.1 - 2 ug/mL

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C in the dark. Avoid freeze-thaw cycles.

Background: ZNFN1A4

Members of the Ikaros (ZNFN1A1; MIM 603023) family of transcription factors, which includes Eos, are expressed in lymphocytes and are implicated in the control of lymphoid development.[supplied by OMIM]

Alternate Names

EOS, IKAROS family zinc finger 4 (Eos), Ikaros family zinc finger protein 4, KIAA1782Eos, zinc finger protein, subfamily 1A, 4 (Eos), zinc finger transcription factor Eos, ZNFN1A4zinc finger protein Eos

Gene Symbol

IKZF4

Additional ZNFN1A4 Products

Product Documents for ZNFN1A4 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ZNFN1A4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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