ZNFN1A4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12160
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Predicted:
Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Peptide with sequence C-RPTFIDRLANSLTKR corresponding to internal region according to NP_071910.3.
Clonality
Polyclonal
Host
Goat
Scientific Data Images for ZNFN1A4 Antibody
Western Blot: ZNFN1A4 Antibody [NBP3-12160]
Western Blot: ZNFN1A4 Antibody [NBP3-12160] - Staining of Jurkat nuclear lysate (A) + Peptide (B) with antibody at 2 ug/mL, and staining of K562 nuclear lysate (C) + Peptide (D) with antibody at 0.1 ug/mL, (35 ug protein in RIPA buffer). Detected by chemiluminescence.Immunocytochemistry/ Immunofluorescence: ZNFN1A4 Antibody [NBP3-12160]
Immunocytochemistry/Immunofluorescence: ZNFN1A4 Antibody [NBP3-12160] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).Flow Cytometry: ZNFN1A4 Antibody [NBP3-12160]
Flow Cytometry: ZNFN1A4 Antibody [NBP3-12160] - Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Applications for ZNFN1A4 Antibody
Application
Recommended Usage
Flow Cytometry
10 ug/mL
Immunocytochemistry/ Immunofluorescence
5 ug/mL
Peptide ELISA
Detection limit 1:2000
Western Blot
0.1 - 2 ug/mL
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C in the dark. Avoid freeze-thaw cycles.
Background: ZNFN1A4
Alternate Names
EOS, IKAROS family zinc finger 4 (Eos), Ikaros family zinc finger protein 4, KIAA1782Eos, zinc finger protein, subfamily 1A, 4 (Eos), zinc finger transcription factor Eos, ZNFN1A4zinc finger protein Eos
Gene Symbol
IKZF4
Additional ZNFN1A4 Products
Product Specific Notices for ZNFN1A4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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